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首页> 外文期刊>Digestive Diseases and Sciences >Estrogen-Induced Proliferation in Cultured Hepatocytes Involves Cyclin D1, P21CIP1 and P27KIP1
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Estrogen-Induced Proliferation in Cultured Hepatocytes Involves Cyclin D1, P21CIP1 and P27KIP1

机译：雌激素诱导的肝细胞增殖涉及细胞周期蛋白D1，P21CIP1 和P27KIP1

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摘要

The purpose of this study was to establish if estrogen-induced hepatocyte proliferation in vitro involves the cell cycle regulators cyclin D1, p21Cip1, and p27Kip1. Male rat hepatocytes were cultured in presence of 17-β -estradiol (E2) ± ICI-182780, a pure estrogen antagonist, and [3H]-thymidine, as required. DNA synthesis as well as p21Cip1, p27Kip1, and cyclin D1mRNA and protein levels were evaluated at different times (12, 24, 36, and 48 hours) of incubation. E2-increased DNA synthesis was correlated with cyclin D1 and p21Cip1 (mRNA and protein) variations that were reversed by the addition of ICI-182780. p27Kip1protein levels progressively increased regardless of the presence of E2 or ICI-182780. Our data confirm that estrogens’ stimulatory effect is related to their ability to increase cyclin D1 levels. The increase of p21Cip1 is probably related to the reentry of hepatocytes in the quiescent state. p27Kip1protein is not able to arrest hepatocyte proliferation.
机译：这项研究的目的是确定雌激素诱导的体外肝细胞增殖是否涉及细胞周期调节因子cyclin D1，p21Cip1 和p27Kip1 。根据需要，在17-β-雌二醇（E2 ）±ICI-182780（一种纯雌激素拮抗剂）和[3H]-胸苷的存在下培养雄性大鼠肝细胞。在孵育的不同时间（12、24、36和48小时）评估了DNA的合成以及p21Cip1 ，p27Kip1 和细胞周期蛋白D1mRNA和蛋白质水平。 E2 增加的DNA合成与细胞周期蛋白D1和p21Cip1 （mRNA和蛋白质）变异相关，这些变异可通过添加ICI-182780来逆转。无论是否存在E2 或ICI-182780，p27Kip1 蛋白的水平都会逐渐升高。我们的数据证实，雌激素的刺激作用与其增加细胞周期蛋白D1水平的能力有关。 p21Cip1 的增加可能与静止状态下肝细胞的再进入有关。 p27Kip1 蛋白不能阻止肝细胞增殖。

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