Fate of Goblet Cells in Experimental Colitis

Mireille K. Makkink; Nicole M.J. Schwerbrock; Michael Mähler; Jos A. Boshuizen; Ingrid B. Renes; Markus Cornberg; Hans J. Hedrich; Alexandra W.C. Einerhand; Hans A. Büller; Siegfried Wagner; Marie-Luise Enss; Jan Dekker

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Fate of Goblet Cells in Experimental Colitis

机译：实验性结肠炎中杯状细胞的命运

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We sought to correlate the characteristic changes in goblet cell morphology in the chronically inflamed large intestine of IL10 −/− mice to specific changes in goblet cell gene expression. In healthy as well as IL10 −/− mice, marked differences were found among the large intestinal regions in goblet cell morphology and gene expression. The mucin Muc2, which is a major determinant of goblet cell morphology, was expressed in most goblet cells, yet only in cells staining positive for both Alcian blue and high iron diamine. TFF3 was expressed in only a small subset of goblet cells. Inflamed colon of IL10 −/− mice still contained high numbers of small, hypotrophic goblet cells with similar histochemical staining and Muc2 and TFF3 expression patterns, contradicting the often reported “goblet cell depletion” in colitis. Quantitatively, the Muc2 and TFF3 levels remained relatively stabile in IL10 −/− mice. Muc2 in distal IL10 −/− colon contained significantly less sulfate residues than in controls, which may compromise its protective properties.

机译：我们试图将慢性发炎的大肠IL10-/-小鼠的杯状细胞形态特征变化与杯状细胞基因表达的特定变化联系起来。在健康小鼠和IL10-/-小鼠中，大肠区域的杯状细胞形态和基因表达存在明显差异。黏蛋白Muc2是杯状细胞形态的主要决定因素，它在大多数杯状细胞中表达，但仅在阿尔辛蓝和高铁二胺染色呈阳性的细胞中表达。 TFF3仅在杯状细胞的一小部分中表达。 IL10-/-小鼠发炎的结肠中仍含有大量具有相似组织化学染色和Muc2和TFF3表达模式的小营养杯状细胞，这与结肠炎中经常报道的“杯状细胞耗竭”相矛盾。在定量上，IL10-/-小鼠中的Muc2和TFF3水平保持相对稳定。 IL10-/-远端结肠中的Muc2所含的硫酸盐残基比对照中的少得多，这可能会损害其保护特性。

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来源
《Digestive Diseases and Sciences》 |2002年第10期|2286-2297|共12页
作者
Mireille K. Makkink; Nicole M.J. Schwerbrock; Michael Mähler; Jos A. Boshuizen; Ingrid B. Renes; Markus Cornberg; Hans J. Hedrich; Alexandra W.C. Einerhand; Hans A. Büller; Siegfried Wagner; Marie-Luise Enss; Jan Dekker;
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作者单位

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children HospitalInstitute for Laboratory Animal Science and Department of Gastroenterology and Hepatology Medical School;

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children Hospital;

Institute for Laboratory Animal Science and Department of Gastroenterology and Hepatology Medical School;

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children Hospital;

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children Hospital;

Department of Gastroenterology and Hepatology Medical School;

Institute for Laboratory Animal Science and Department of Gastroenterology and Hepatology Medical School;

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children Hospital;

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children Hospital;

Department of Gastroenterology and Hepatology Medical School;

Institute for Laboratory Animal Science and Department of Gastroenterology and Hepatology Medical School;

Paediatric Gastroenterology and Nutrition Department of Paediatrics Erasmus University and Sophia Children Hospital;

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正文语种 eng
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goblet cell; Muc2; sulfation; TFF3; carbonic anhydrase 1;

机译：杯状细胞;Muc2;硫酸化;TFF3;碳酸酐酶1;

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Fate of Goblet Cells in Experimental Colitis

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