• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>DNA and Cell Biology >Rat Protein Kinase C Zeta Gene Contains Alternative Promoters for Generation of Dual Transcripts with 5′-End Heterogeneity
【24h】

Rat Protein Kinase C Zeta Gene Contains Alternative Promoters for Generation of Dual Transcripts with 5′-End Heterogeneity

机译：大鼠蛋白激酶C Zeta基因包含替代启动子，用于产生具有5'-末端异质性的双转录本

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Protein kinase C (PKC) ζ is a phospholipid-dependent serine/threonine kinase that appears to perform important cell signaling functions. Two forms of PKC ζ RNA, with different 5′ ends, have been reported. The major form (ζ) is expressed in most, if not all tissues, while the minor form (ζ′), which encodes the catalytic domain of the enzyme without most of its regulatory domain, is predominant in normal brain and certain rat prostate tumors. We report here the structure of the 5′ end of the rat PKC ζ gene, demonstrating that both forms of RNA can be transcribed from the same gene through the use of alternative promoters and splicing. In luciferase reporter constructs, progressive deletions of the PKC ζ and ζ′ 5′ flanking sequences yielded activities that were higher in the cell lines expressing endogenous PKC ζ and ζ′ RNAs, respectively. Also, multiple PCRs across different introns of the PKC ζ gene indicate that recent duplication of the gene or the existence of a closely related pseudogene in the rat genome are unlikely.
机译：蛋白激酶C（PKC）ζ是一种磷脂依赖性丝氨酸/苏氨酸激酶，似乎具有重要的细胞信号传导功能。已经报道了两种形式的具有不同5'端的PKCζRNA。主要形式（ζ）在大多数（如果不是全部）组织中表达，而次要形式（ζ'）在正常脑和某些大鼠前列腺肿瘤中占主导地位，该次要形式编码酶的催化结构域而没有大部分调节域。我们在这里报告了大鼠PKCζ基因5'端的结构，表明可以通过使用替代启动子和剪接从同一基因转录两种形式的RNA。在萤光素酶报告基因构建物中，PKCζ和ζ'5'侧翼序列的逐步缺失产生的活性分别在表达内源性PKCζ和ζ'RNA的细胞系中更高。同样，跨越PKCζ基因不同内含子的多次PCR表明，该基因的近期复制或大鼠基因组中不存在紧密相关的假基因是不可能的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号