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首页> 外文期刊>Doklady biochemistry & biophysics >Magnetic Labeling of Proteins for Atomic Force Microscopy
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Magnetic Labeling of Proteins for Atomic Force Microscopy

机译：蛋白质的磁性标记，用于原子力显微镜

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摘要

Today, biological objects are studied using various microscopy methods: electronic microscopy (EM), confocal microscopy (CM), and atomic force microscopy (AFM). One of the advantages of AFM is the ability to study biological objects in solution at a resolution comparable to that of electron microscopy. In EM and CM, there are methods based on the use of antibodies conjugated to metals or fluorophores to identify specific proteins in the study object. In AFM, there is currently no convenient, versatile method to introduce a specific label. In this paper, we propose identification of proteins in complex samples by using magnetic particles conjugated to antibodies. Imaging of the spatial distribution of the z-component of the gradient of magnetic forces on the surface of the sample makes it possible to identify the spatial position of protein structures. As a model for developing such a system, we used abnormal fibrils of human a-lactalbu-min immobilized on a substrate and iron oxide nano-particles functionalized with antibodies to this protein.
机译：如今，使用各种显微镜方法研究生物对象：电子显微镜（EM），共聚焦显微镜（CM）和原子力显微镜（AFM）。 AFM的优点之一是能够以与电子显微镜相当的分辨率研究溶液中的生物对象。在EM和CM中，存在基于使用与金属或荧光团偶联的抗体来鉴定研究对象中特定蛋白质的方法。在AFM中，目前没有方便，通用的方法来引入特定标签。在本文中，我们建议使用与抗体偶联的磁性颗粒鉴定复杂样品中的蛋白质。样品表面上磁力梯度的z分量的空间分布成像可以识别蛋白质结构的空间位置。作为开发这种系统的模型，我们使用了固定在基质上的人α-乳清蛋白的异常原纤维和用针对该蛋白的抗体功能化的氧化铁纳米颗粒。

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  • 来源
    《Doklady biochemistry & biophysics》 |2013年第janaafeba期|33-35|共3页
  • 作者

    V. V. Egorov; Y. A. Zabrodskaya; A. S. Kalinin; D. V. Lebedev; N. A. Grudinina; A. V. Vasin; V. A. Kolikov; S. A. Klotchenko; M. M. Shawlovsky; Ph. G. Rutberg;

  • 作者单位

    Influenza Research Institute, Russian Academyof Medical Sciences, ul. Professora Popova 15/17,St. Petersburg, 197376 Russia;

    Influenza Research Institute, Russian Academyof Medical Sciences, ul. Professora Popova 15/17,St. Petersburg, 197376 Russia;

    ZAO NT-MDT, korp. 100, Zelenograd, Moscow,124482 Russia;

    Konstantinov Institute of Nuclear Physics, Russian Academy of Sciences, Gatchina, Leningradskaya oblast, 188300 Russia;

    Institute of Experimental Medicine, Russian Academy of Medical Sciences, ul. Akademika Pavlova 12,St. Petersburg, 197376 Russia;

    Influenza Research Institute, Russian Academyof Medical Sciences, ul. Professora Popova 15/17,St. Petersburg, 197376 Russia;

    ZAO NT-MDT, korp. 100, Zelenograd, Moscow,124482 Russia;

    Influenza Research Institute, Russian Academyof Medical Sciences, ul. Professora Popova 15/17,St. Petersburg, 197376 Russia;

    Institute of Experimental Medicine, Russian Academy of Medical Sciences, ul. Akademika Pavlova 12,St. Petersburg, 197376 Russia;

    Institute of Electrophysics and Electric Power,Russian Academy of Sciences, Dvortsovaya nab. 18,St. Petersburg, 191186 Russia;

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