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首页> 外文期刊>Ecological research >Seasonal variations in PCR-DGGE fingerprinted viruses infecting phytoplankton in large and deep peri-alpine lakes
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Seasonal variations in PCR-DGGE fingerprinted viruses infecting phytoplankton in large and deep peri-alpine lakes

机译：在大和深的高山湖泊中感染浮游植物的PCR-DGGE指纹图谱病毒的季节性变化

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Double-stranded DNA viruses infecting eukaryotic algae (e.g., phycodnaviruses) and cyanobacte-ria (e.g., cyanophages) are now recognized as widespread and ubiquitous in aquatic environments. However, both the diversity and functional roles of these viruses in fresh waters are still poorly understood. We conducted a yearlong study in 2011 of the community structure of plank-tonic virus groups in the upper lit layer of two important freshwater natural ecosystems in France, Lake Annecy (oligotrophic) and Lake Bourget (oligo-mesotrophic). Using PCR-DGGE to target a number of different structural and functional signature genes, i.e.,g20, g23, psbA, polB, and mcp, the phytoplankton viruses were shown to display temporal and spatial variability. There were marked seasonal changes in community structure for all viral groups in Lake Bourget, but only for T4-like my-oviruses and psb-containing cyanophages in Lake Annecy. The multivariate statistical analyses revealed that (1) various environmental factors can directly or indirectly explain the community structure observed for each phytoplankton viral group, and (2) temporal patterns of T4-like myovirus community structure were similar between the two lakes. In general, our results (1) suggest that the observed algal virus patterns were associated with significant shifts in phytoplankton biomass and/or structure, ', which in turn were shaped by the abiotic environment, and (2) support the Bank model proposed by Breitbart and Rohwer (Trends Microbiol 13:278-284, 2005). This study provides new evidence that freshwater lakes contain a ; significant diversity of algal viruses, and that the distribution of these viruses strongly mirrors that of their hosts.
机译：现在已经认识到，感染真核藻类（例如，phycodnaviruses）和蓝细菌-ria（例如，噬菌体）的双链DNA病毒在水生环境中是普遍存在的。但是，这些病毒在淡水中的多样性和功能作用仍然知之甚少。我们在2011年对法国两个重要的淡水自然生态系统上浅层上的浮游生物病毒群的群落结构进行了为期一年的研究，这两个自然淡水生态系统是法国的安纳西湖（贫营养型）和布尔热湖（中营养的低级）。使用PCR-DGGE靶向许多不同的结构和功能签名基因，即g20，g23，psbA，polB和mcp，浮游植物病毒显示出时空变异性。布尔热湖中所有病毒组的群落结构都有明显的季节性变化，但仅在安纳西湖中的T4样肌病毒和含psb的噬菌体才有。多元统计分析显示，（1）各种环境因素可以直接或间接解释每个浮游植物病毒组观察到的群落结构，（2）两个湖泊之间的T4样肌病毒群落结构的时间模式相似。一般而言，我们的结果（1）表明所观察到的藻类病毒模式与浮游植物生物量和/或结构的显着变化有关，而这些变化又受非生物环境的影响，并且（2）支持由浮游生物提出的Bank模型。 Breitbart和Rohwer（Trends Microbiol 13：278-284，2005）。这项研究提供了新的证据，表明淡水湖泊包含了淡水湖泊;藻类病毒的多样性非常明显，而且这些病毒的分布强烈反映了其宿主的分布。

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