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首页> 外文期刊>Ecological research >Intra-population variation and geographic correlation in Canthon humectus hidalgoensis using FTIR-ATR spectroscopy
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Intra-population variation and geographic correlation in Canthon humectus hidalgoensis using FTIR-ATR spectroscopy

机译:FTIR-ATR光谱分析Hidectus hidalgoensis的种群内变异和地理相关性

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摘要

In some cases external morphology is not sufficient to discern between populations of a species, as occurs in the dung beetle Canthon humectus hidalgoensis Bates; and much less to determine phenotypic distances between them. FTIR-ATR spectroscopy show several advantages over other identification techniques (e.g. morphological, genetic, and cuticular hydrocarbons analysis) due to the non-invasive manner of the sample preparation, the relative speed of sample analysis and the low-cost of this technology. The infrared spectrum obtained is recognized to give a unique 'fingerprint' because vibrational spectra are specific and unique to the molecular nature of the sample. In our study, results showed that proteins, amino acids and aromatic ethers of insect exocuticle have promising discriminative power to discern between different populations of C. h. hidalgoensis. Furthermore, the correlation between geographic distances between populations and the chemical distances obtained by proteins + amino acids + aromatic ethers was statistically significant, showing that the spectral and spatial information available of the taxa together with appropriated chemometric methods may help to a better understanding of the identity, structure, dynamics and diversity of insect populations.
机译:在某些情况下,外部形态不足以区分物种的种群,就像在甲虫Canthon humectus hidalgoensis Bates中所发生的那样。而确定它们之间的表型距离则少得多。 FTIR-ATR光谱显示出比其他识别技术(例如形态,遗传和表皮碳氢化合物分析)更多的优势,这归因于样品制备的非侵入性方式,样品分析的相对速度以及该技术的低成本。所获得的红外光谱被认为具有独特的“指纹”,因为振动光谱是特定的并且对于样品的分子性质而言是独特的。在我们的研究中,结果表明,昆虫表皮的蛋白质,氨基酸和芳香族醚具有有前途的区分能力,以区分不同的中华绒螯蟹。 hidalgoensis。此外,种群之间的地理距离与蛋白质+氨基酸+芳香族醚获得的化学距离之间的相关性具有统计学意义,表明分类单元可获得的光谱和空间信息以及适当的化学计量方法可能有助于更好地了解昆虫种群的身份,结构,动态和多样性。

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