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首页> 外文期刊>Ecological research >Trophic position estimates of formalin-fixed samples with nitrogen isotopic compositions of amino acids: an application to gobiid fish (Isaza) in Lake Biwa, Japan
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Trophic position estimates of formalin-fixed samples with nitrogen isotopic compositions of amino acids: an application to gobiid fish (Isaza) in Lake Biwa, Japan

机译：具有氨基酸的氮同位素组成的福尔马林固定样品的营养位置估计：在日本琵琶湖中的戈壁鱼（Isaza）中的应用

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摘要

To evaluate the influence of formalin-fixation on the nitrogen isotopic composition of individual amino acids, we conducted a formalin-fixation experiment over 62 weeks using muscle tissues of largemouth bass (Micropterus salmoides) and freshwater minnow (Zacco platypus) collected in Lake Biwa. The results indicated that the formalin-fixation does not affect the nitrogen isotopic composition of amino acids including glutamic acid and phenylalanine from muscle protein of the fish. We applied this technique to formalin-fixed gobiid fish "Isaza" (Gymnogobius isaza, Tanaka) specimens that had been collected from Lake Biwa through the twentieth century. The pattern of the (δ~(15)N values for these amino acids remains relatively constant, even though each amino acid exhibits a gradual increase by ～3 ‰ from 1916 to 1992. With a formula proposed by Chikaraishi et al. (Limnol Oceanogr Method 7:740-750, 2009), we estimated the temporal variations in the trophic position of Isaza in Lake Biwa. The trophic position of Isaza remained quite constant (3.2-3.3) across a major eutrophication period in 1960-1980. The estimated trophic position of Isaza fish is consistent with the stomach content analysis in the previous reports. The constant trophic position suggests that the eutrophication did not apparently affect the trophic position of Isaza fish, although Isaza's dietary preference changed from zooplankton to gammarids around 1970.
机译：为了评估福尔马林固定对单个氨基酸的氮同位素组成的影响，我们使用琵琶湖中收集的大嘴鲈（Micropterus salmoides）和淡水min鱼（Zacco platypus）的肌肉组织进行了62周的福尔马林固定实验。结果表明，福尔马林固定不影响鱼类肌肉蛋白中谷氨酸和苯丙氨酸等氨基酸的氮同位素组成。我们将此技术应用于二十世纪从琵琶湖采集的福尔马林固定戈壁鱼“ Isaza”（田中Gymnogobius isaza）标本中。这些氨基酸的（δ〜（15）N值的模式保持相对恒定，即使每个氨基酸在1916年至1992年之间都逐渐增加〜3‰。使用Chikaraishi等人提出的公式（Limnol Oceanogr方法7：740-750，2009），我们估算了琵琶湖Isaza的营养位置随时间的变化，在1960-1980年的主要富营养化时期，Isaza的营养位置保持相当恒定（3.2-3.3）。伊萨扎鱼的营养位置与以前的报道中的胃液含量分析一致，尽管伊萨扎的饮食偏爱从浮游动物变为伽马s鱼，但营养位置不变表明富营养化对伊萨扎鱼的营养位置没有明显影响。

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  • 来源
    《Ecological research》 |2013年第5期|697-702|共6页
  • 作者

    Nanako O. Ogawa; Y. Chikaraishi; N. Ohkouchi;

  • 作者单位

    Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Department of Environmental Chemical Engineering, Tokyo Institute of Technology, Tokyo, Japan Department of Earth and Planetary Science, University of Tokyo, Tokyo, Japan;

    Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan;

    Institute of Biogeosciences, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan;

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  • 正文语种 eng
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  • 关键词

    Nitrogen isotope ratio; Amino acid; Formalin fixation; Trophic position;

    机译：氮同位素比;氨基酸;福尔马林固定营养位置;

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