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首页> 外文期刊>Ecological research >Simple nonlethal identification criteria for two endangered freshwater pearl mussels, Margaritifera laevis and Margaritifera togakushiensis, in Hokkaido, northern Japan
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Simple nonlethal identification criteria for two endangered freshwater pearl mussels, Margaritifera laevis and Margaritifera togakushiensis, in Hokkaido, northern Japan

机译:日本北部北海道的两种濒临灭绝的淡水珍珠贻贝,玛格丽塔酒和玛格丽塔酒的简单非致死鉴定标准

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摘要

Species identification is a fundamental process for ecological studies and conservation practices, and simple nonlethal identification criteria are important for endangered species. This study developed species identification criteria for two endangered freshwater pearl mussels (Margaritifera laevis and Margaritifera togakushiensis) based on linear discriminant functions (LDFs) that were established considering intraspecific regional morphological differences from sites at Hokkaido, northern Japan. We collected a total of 1,110 mussels from 52 rivers across 32 watersheds in two geographical regions (east and west). Shell morphologies (length, height, and width) of the collected mussels were measured, followed by species identification with gel electrophoresis banding patterns of 16S rRNA polymerase chain reaction products. LDFs were constructed for two size classes (>= 40mm and<40mm) using forward stepwise procedures to determine key morphological differences between the two species and consideration of whether regional morphological differences improved identification accuracies. The LDFs revealed that the morphologies of the two species were clearly distinguished by the relationship between height and length for both size classes. Region-specific LDFs produced better identification accuracies. Regardless of region, the maximum length of M. laevis exceeded 100mm, whereas all M. togakushiensis were<100mm in length. Identification accuracies of the established LDFs were high for each of the five length classes (0-20mm, 20-40mm, 40-60mm, 60-80mm, and 80-100mm) with 85-96% (mean: 92%) accuracy in the east and 67-96% (mean: 80%) in the west. These criteria for species identification will progress future ecological studies and conservation practices for freshwater mussels.
机译:物种识别是生态学研究和保护实践的基本过程,简单的非致死性识别标准对濒危物种很重要。这项研究基于线性判别函数(LDF)建立了两个濒临灭绝的淡水珍珠贻贝(Margaritifera laevis和Margaritifera togakushiensis)的物种鉴定标准,该线性判别函数是考虑到日本北部北海道地区种内区域形态差异而建立的。我们从两个地理区域(东部和西部)的32个流域中的52条河流中收集了总计1,110只贻贝。测量收集到的贻贝的壳形态(长度,高度和宽度),然后使用16S rRNA聚合酶链反应产物的凝胶电泳条带模式进行物种鉴定。使用逐步步骤确定两个物种(> = 40mm和<40mm)的LDF,以确定两个物种之间的关键形态差异,并考虑区域形态差异是否提高了识别准确性。 LDF揭示了两个物种的形态通过两个尺寸类别的高度和长度之间的关系清楚地区分。特定于区域的LDF产生了更好的识别准确性。无论区域如何,La。Laevis的最大长度都超过100mm,而togakushiensis的所有长度都小于100mm。在五个长度等级(0-20mm,20-40mm,40-60mm,60-80mm和80-100mm)中,已建立的LDF的识别精度均很高,其精度为85-96%(平均:92%)。东部为67-96%(平均80%)。这些物种鉴定标准将促进未来淡水贻贝的生态研究和保护实践。

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