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首页> 外文期刊>Ecotoxicology and Environmental Safety >Annona muricata: An alternate mosquito control agent with special reference to inhibition of detoxifying enzymes in Aedes aegypti
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Annona muricata: An alternate mosquito control agent with special reference to inhibition of detoxifying enzymes in Aedes aegypti

机译：Annona muricata：一种替代性的蚊香控制剂，特别用于抑制埃及伊蚊的解毒酶

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摘要

This study was aimed to investigate an effectual level of Annona muricata (soursop) extracts on mosquito vectors namely, Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus. The toxicity study on non-target organism and other important biochemical marker enzymes to find and illustrate the exact mechanism of specific enzymes responsible for detoxifying allelochemicals. Among the various soursop seed kernel extracts tested for larvicidal activity, the 0.9% saline extract exhibited maximum mortality (100%) against three vectors at the lowest concentration for 24 h exposure. Based on these findings, the saline extract was opted for further studies including toxicity on non-target organism and systemic effects on important biochemical constituents in the larvae A. aegypti at the lethal threshold time (18 h) with LC50 concentration (0.009 mg/mL). The tested extract against non-target aquatic fourth instar larvae Chironomus costatus was safe up to 0.0028 mg/mL for 24 h exposure and the mortality was observed only above the concentration 0.0028 mg/mL used in the study. The systemic effects on main neuron transmitter Acetylcholinesterase (p <= 0.01), xenobiotics detoxifying enzyme of alpha-and beta-carboxylesterase (p <= 0.05; p <= 0.01) and antioxidant enzyme glutathione S-transferase (p <= 0.05) were reduced significantly in quantitative analysis. Analysis of such biochemical constituents of proteins and enzymes alpha-and beta-carboxylesterase were considerably down regulated in the resolving native-PAGE. In contrast, acid and alkaline phosphatase were upregulated in both quantitative and qualitative analysis. This investigation clearly demonstrates the soursop extract has potent larvicidal agent with alterations in biochemical constituents of exposed larvae of A. aegypti.
机译：这项研究旨在调查番荔枝番荔枝提取物对蚊媒（埃及伊蚊，按蚊按蚊和库蚊）的有效水平。对非靶标生物和其他重要生化标记酶的毒性研究，以发现并阐明负责化感化学物质解毒的特定酶的确切机理。在各种测试的杀幼虫活性的刺五加籽仁提取物中，0.9％的盐提取物在24 h最低浓度下对三种载体表现出最大的死亡率（100％）。基于这些发现，我们选择了盐提取物进行进一步研究，包括对非靶标生物的毒性以及对埃及A幼虫在致死阈值时间（18 h）内LC50浓度（0.009 mg / mL）的重要生化成分的系统性作用）。测试的针对非目标水生四龄幼虫Chironomus costatus的提取物在暴露24 h时高达0.0028 mg / mL是安全的，并且仅在研究中使用的0.0028 mg / mL以上才观察到死亡率。对主要神经元递质乙酰胆碱酯酶（p <= 0.01），α-和β-羧酸酯酶的异种生物解毒酶（p <= 0.05; p <= 0.01）和抗氧化酶谷胱甘肽S-转移酶（p <= 0.05）的全身作用为定量分析显着降低。蛋白质和酶α-和β-羧酸酯酶的此类生化成分的分析在解析的native-PAGE中显着下调。相反，在定量和定性分析中，酸性和碱性磷酸酶均上调。这项研究清楚地证明了刺五加提取物具有有效的杀幼虫剂，而埃及伊蚊的幼虫的生化成分发生了变化。

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