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首页> 外文期刊>Ecotoxicology and Environmental Safety >Bioluminescent method for the rapid screening of toxic heayy metals in environmental samples using Photobacterium leiognathi strain AK-MIE
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Bioluminescent method for the rapid screening of toxic heayy metals in environmental samples using Photobacterium leiognathi strain AK-MIE

机译：使用光杆菌Leioghathi菌株Ak-mie快速筛选在环境样品中有毒的HEAYY金属的快速筛选方法

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摘要

Assessment of eco-toxicant using bioluminescent bacterial assay is a widely used and globally accepted method. In this work, a new luminescent bacterium was isolated from squid (Loligo duvauceli) and identified as Photobacterium leiognathi strain AK-MIE using 16S rRNA, phylogeny analysis. The predicted optimum conditions by RSM were 2.76% (w/v) NaCl, 2.28% (w/v) peptone, 0.34% (w/v) yeast extract, and pH 6.83 with 541,211.80 RLU of luminescent production whereas the predicted optimum conditions by ANN were 2.21% (w/v) NaCl, 2.27% (w/v) peptone, 0.39% (w/v) yeast extract, and pH 6.94 which produced 541,986.20 RLU. The validation analysis of both RSM and ANN show 0.60% and 0.69% deviation from the predicted results indicating that both models provided good quality predictions with ANN showing a superior data fitting capability for non-linear regression analysis. Toxicity tests show strain AK-MIE was sensitive to mercury (concentration causing 50% inhibition or IC50 of 0.00978 mgL(-1)), followed by cadmium (IC50 of 0.5288 mgL(-1)), copper IC50 of (0.8117 mgL(-1)), silver (IC50 of 1.109 mgL(-1)), and lead (IC50 of 10.71 mgL(-1)) which are more sensitive than previously isolated luminescent bacteria, suggesting that strain AK-MIE has the potential to be used in toxicity assessment of heavy metals in the environment. Based on the field trial results, several sediment samples from industrial areas in Bangi, Selangor managed to inhibit the bioluminescence of strain AK-MIE. Validation method carried out using ICP-MS proved the presence of several toxic heavy metal elements.
机译：使用生物发光细菌测定评估生态毒物是广泛使用的和全球接受的方法。在这项工作中，从鱿鱼（Loligo Duvauceli）中分离出一种新的发光细菌，并使用16S rRNA，系统发育分析鉴定为Pherogacterium Leiognathi菌株Ak-mie。 RSM预测的最佳条件为2.76％（w / v）NaCl，2.28％（w / v）蛋白胨，0.34％（w / v）酵母提取物，pH 6.83，发光生产541,211.80 rlu，而预测的最佳条件Ann为2.21％（w / v）NaCl，2.27％（w / v）蛋白胨，0.39％（w / v）酵母提取物，pH 6.94，其生产541,986.20 rlu。 RSM和ANN的验证分析显示了从预测结果的偏差0.60％和0.69％，表明两种模型都提供了良好的质量预测，并显示了非线性回归分析的卓越数据拟合能力。毒性试验显示菌株Ak-mie对汞敏感（浓度导致50％抑制或IC50的0.00978mg（-1）），其次是镉（IC50为0.5288mg1），铜IC50（0.8117 mg1）（0.8117 mg1）（ - 1）），银（IC50为1.109 mg（-1）），铅（IC50为10.71 mg1），其比以前分离的发光细菌更敏感，表明菌株Ak-mie有可能使用毒性评估环境中重金属。基于现场试验结果，林兰工业区的几个沉积物样本，雪兰莪群组成功地抑制了菌株Ak-mie的生物发光。使用ICP-MS进行的验证方法证明存在几种有毒重金属元素。

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