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首页> 外文期刊>Ecotoxicology and Environmental Safety >Development of in vivo biotransformation enzyme assays for ecotoxicity screening: In vivo measurement of phases I and II enzyme activities in freshwater planarians
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Development of in vivo biotransformation enzyme assays for ecotoxicity screening: In vivo measurement of phases I and II enzyme activities in freshwater planarians

机译：用于生态毒性筛查的体内生物转化酶测定方法的开发：淡水涡卷虫体内I和II期酶活性的体内测量

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The development of a high-throughput tool is required for screening of environmental pollutants and assessing their impacts on aquatic animals. Freshwater planarians can be used in rapid and sensitive toxicity bioassays. Planarians are known for their remarkable regeneration ability but much less known for their metabolic and xenobiotic biotransformation abilities. In this study, the activities of different phase I and II enzymes were determined in vivo by directly measuring fluorescent enzyme substrate disappearance or fluorescent enzyme metabolite production in planarian culture media. For phase I enzyme activity, O-deethylation activities with alkoxyresorufin could not be detected in planarian culture media. By contrast, O-deethylation activities with alkoxycoumarin were detected in planarian culture media. Increases in 7-ethoxycoumarin O-deethylase (ECOD) activities was only observed in planarians exposed to 1 mu M, but not 10 mu M, beta-naphthoflavone for 24 h. ECOD activity was inhibited in planarians exposed to 10 and 100 mu M rifampicin or carbamazepine for 24 h. For phase II enzyme activity, DT-diaphorase, arylsulfatases, uridine 5'-diphospho (UDP)-glucuronosyltransferase or catechol-O-methyltransferase activity was determined in culture media containing planarians. The results of this study indicate that freshwater planarians are a promising model organism to monitor exposure to environmental pollutants or assess their impacts through the in vivo measurement of phase I and II enzyme activities. (C) 2016 Published by Elsevier Inc.
机译：需要开发一种高通量的工具来筛选环境污染物并评估其对水生动物的影响。淡水涡虫可以用于快速和敏感的毒性生物测定。平面动物以其卓越的再生能力而闻名，但其代谢和异源生物转化能力却鲜为人知。在这项研究中，通过直接测量荧光素培养基中荧光酶底物的消失或荧光酶代谢产物的产生，在体内确定了不同的I和II期酶的活性。对于I期酶活性，在涡虫培养基中无法检测到具有烷氧基间苯二酚的O-脱乙基活性。相比之下，在涡虫培养基中检测到烷氧基香豆素的O-脱乙基活性。仅在暴露于1μM而不是10μMβ-萘黄酮持续24 h的平面动物中观察到7-乙氧基香豆素O-脱乙基酶（ECOD）活性的增加。 ECOD活性在暴露于10和100μM利福平或卡马西平达24小时的涡虫中被抑制。对于II期酶活性，在含有平面虫的培养基中测定了DT-黄递酶，芳基硫酸酯酶，尿苷5'-二磷酸（UDP）-葡萄糖醛糖基转移酶或邻苯二酚-O-甲基转移酶的活性。这项研究的结果表明，淡水浮游动物是一种有前途的模型生物，可以监测环境污染物的暴露或通过体内测量I和II期酶的活性来评估其影响。 （C）2016由Elsevier Inc.发布

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