...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Ecotoxicology and Environmental Safety >In vivo and in vitro genotoxicity assessment of 2-methylisoborneol, causal agent of earthy-musty taste and odor in water
【24h】

In vivo and in vitro genotoxicity assessment of 2-methylisoborneol, causal agent of earthy-musty taste and odor in water

机译：2-甲基异冰片酚的体内和体外遗传毒性评估，水为泥土味和臭味的病因

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The water eutrophication process by phosphorus and nitrogen allows cyanobacteria blooms which promote, among other effects, the generation and release of the metabolite 2-methylisoborneol (2-MIB) in the environment. This substance has been shown to be recalcitrant to conventional water treatment, degrading water quality. Considering the limited number of studies on the biological effects of 2-MIB in eukaryotic organisms, the present study assessed the genotoxicity of 2-MIB using the in vitro comet assay and cytokinesis block-micronucleus (CBMN-Cytome) assay on Chinese Hamster Ovary (CHO) cells and the in vivo Drosophila melanogaster Somatic Mutation and Recombination Test (SMART). The results showed that 2-MIB (125, 250 and 500 μg/mL) was unable to induce gene and chromosome mutations or events associated with mitotic recombination in the SMART. Similarly, four different concentrations (7.5, 15, 30 and 60 μg/mL) of 2-MIB did not induce increments in frequencies of micronuclei, nuclear buds, and nucleoplasmatic bridges in the CBMN-Cytome assay. In the comet assay, the positive results were restricted to the highest dose, 60 μg/mL of 2-MIB. The results obtained may help evaluate the genotoxic profile of extracellular algal products.
机译：磷和氮引起的水富营养化过程使蓝细菌大量繁殖，除其他作用外，还促进了环境中代谢物2-甲基异冰片醇（2-MIB）的产生和释放。该物质被证明对常规水处理有害，会降低水质。考虑到关于2-MIB在真核生物中生物学作用的研究数量有限，本研究使用体外彗星试验和胞质分裂微核（CBMN-Cytome）试验对中国仓鼠卵巢（2-C）的遗传毒性进行了评估。 CHO）细胞和体内果蝇果蝇体细胞突变和重组试验（SMART）。结果表明，2-MIB（125、250和500μg/ mL）无法诱导SMART中的基因和染色体突变或与有丝分裂重组相关的事件。类似地，在CBMN-Cytome分析中，四种不同浓度的（2-7.5 mg / mL，分别为7.5、15、30和60μg/ mL）不会引起微核，核芽和核质桥的频率增加。在彗星试验中，阳性结果仅限于最高剂量的2-MIB 60μg/ mL。获得的结果可能有助于评估细胞外藻类产品的遗传毒性。

著录项

  • 来源
    《Ecotoxicology and Environmental Safety》 |2014年第2期|282-286|共5页
  • 作者

    Leonel Burgos; Mauricio Lehmann; Heloisa Helena Rodrigues de Andrade; Bianca Regina Ribas de Abreu; Ana Paula de Souza; Viviane Berwanger Juliano; Rafael Rodrigues Dihl;

  • 作者单位

    Laboratorio de Toxicidade Genetica, Programa de Pos-Graduacao em Biologia Celular e Molecular Aplicada a Saude (PPGBioSaude), ULBRA, Canoas, RS, Brazil;

    Laboratorio de Toxicidade Genetica, Programa de Pos-Graduacao em Biologia Celular e Molecular Aplicada a Saude (PPGBioSaude), ULBRA, Canoas, RS, Brazil;

    Laboratorio de Estomatologia, Hospital de Clinicas de Porto Alegre, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil;

    Laboratorio de Toxicidade Genetica, Programa de Pos-Graduacao em Biologia Celular e Molecular Aplicada a Saude (PPGBioSaude), ULBRA, Canoas, RS, Brazil;

    Laboratorio de Toxicidade Genetica, Programa de Pos-Graduacao em Biologia Celular e Molecular Aplicada a Saude (PPGBioSaude), ULBRA, Canoas, RS, Brazil;

    Institute de Pesquisas Hidraulicas (IPH), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil;

    Laboratorio de Toxicidade Genetica, Programa de Pos-Graduacao em Biologia Celular e Molecular Aplicada a Saude (PPGBioSaude), ULBRA, Canoas, RS, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    2-MIB; SMART; Comet assay; CBMN; Genetic toxicity;

    机译：2-MIB;聪明;彗星试验;CBMN;遗传毒性;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号