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首页> 外文期刊>ELECTROPHORESIS >The application of magnetic bead hybridization for the recovery and STR amplification of degraded and inhibited forensic DNA†
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The application of magnetic bead hybridization for the recovery and STR amplification of degraded and inhibited forensic DNA†

机译：磁珠杂交技术在降解和抑制法医DNA回收和STR扩增中的应用†

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摘要

A common problem in the analysis of forensic DNA evidence is the presence of environmentally degraded and inhibited DNA. Such samples produce a variety of interpretational problems such as allele imbalance, allele dropout and sequence specific inhibition. In an attempt to develop methods to enhance the recovery of this type of evidence, magnetic bead hybridization has been applied to extract and preconcentrate DNA sequences containing short tandem repeat (STR) alleles of interest. In this work, genomic DNA was fragmented by heating, and sequences associated with STR alleles were selectively hybridized to allele-specific biotinylated probes. Each particular biotinylated probe–DNA complex was bound to streptavidin-coated magnetic beads using enabling enrichment of target DNA sequences. Experiments conducted using degraded DNA samples, as well as samples containing a large concentration of inhibitory substances, showed good specificity and recovery of missing alleles. Based on the favorable results obtained with these specific probes, this method should prove useful as a tool to improve the recovery of alleles from degraded and inhibited DNA samples.
机译：法医DNA证据分析中的一个常见问题是存在环境降解和抑制的DNA。这样的样品产生各种解释性问题，例如等位基因失衡，等位基因缺失和序列特异性抑制。在尝试开发增强此类证据回收的方法的过程中，磁珠杂交技术已应用于提取和预浓缩含有目标短串联重复（STR）等位基因的DNA序列。在这项工作中，通过加热使基因组DNA断裂，并将与STR等位基因相关的序列选择性地与等位基因特异性生物素化探针杂交。每个特定的生物素化探针-DNA复合物都可以通过使目标DNA序列富集而与抗生蛋白链菌素包被的磁珠结合。使用降解的DNA样品以及含有大量抑制物质的样品进行的实验显示出良好的特异性和缺失等位基因的回收率。基于这些特异性探针获得的良好结果，该方法应被证明可作为一种工具，用于提高降解和抑制的DNA样品中等位基因的回收率。

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