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Characterization of heterocystous cyanobacterial strains for biodiesel production based on fatty acid content analysis and hydrocarbon production

机译:基于脂肪酸含量分析和碳氢化合物生产的用于生物柴油生产的杂藻蓝细菌菌株的表征

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摘要

The present investigation evaluates the potential of fifteen heterocystous cyanobacterial strains for biodiesel and hydrocarbon production. Fatty acid composition, biodiesel qualities along with biomass, lipid and hydrocarbon productivity were determined under autotrophic growth conditions. The biomass productivity of tested cyanobacterial strains ranged from 8.25 to 24.39 mg L-1 day(-1) dry cell weight (DCW), whereas the lipid and hydrocarbon productivity ranged from 0.747 to 3.259 mg L-1 day(-1) and 0.153 to 0.516 mg L-1 day(-1), respectively. Palmitic acid (3.79-40.84%) and stearic acid (14.64-69.84%), which are favourable for biodiesel production, were predominantly present in tested cyanobacterial strains. Biodiesel fuel properties were estimated by empirical formulas based on FAME composition and met the prescribed criteria of international biodiesel standards like ASTM D6751 in the United States, EN 14214 in Europe and IS 15607 in India. Nostoc calcicoia MBDU 602, with a lipid content of 18.921 +/- 0.096 (% dwt), lipid productivity of 3.259 +/- 0.211 mg L-1 day(-1) and desirable fatty acid composition of C16-C18 (57.78%) was selected as the most suitable species for biodiesel production through PROMETHEE-GAIA analysis. The selected strain could possibly be exploited at commercial scale after further evaluation of up- and down-stream process at pilot experiment using photo-bioreactors.
机译:本研究评估了十五种异囊藻蓝细菌菌株在生物柴油和碳氢化合物生产中的潜力。在自养生长条件下测定脂肪酸组成,生物柴油质量以及生物量,脂质和烃的生产率。经测试的蓝细菌菌株的生物量生产力范围为8.25至24.39 mg L-1天(-1)干细胞重量(DCW),而脂质和碳氢化合物生产力范围为0.747至3.259 mg L-1天(-1)和0.153至0.516 mg L-1天(-1)。对生物柴油生产有利的棕榈酸(3.79-40.84%)和硬脂酸(14.64-69.84%)主要存在于测试的蓝细菌菌株中。通过基于FAME组成的经验公式估算生物柴油燃料的性能,并符合国际生物柴油标准的规定标准,例如美国的ASTM D6751,欧洲的EN 14214和印度的IS 15607。 Nostoc calcicoia MBDU 602,脂质含量为18.921 +/- 0.096(%dwt),脂质生产率为3.259 +/- 0.211 mg L-1天(-1),所需的C16-C18脂肪酸组成(57.78%)通过PROMETHEE-GAIA分析,它被选为最适合生物柴油生产的物种。在使用光生物反应器进行的中试实验中,进一步评估上游和下游过程后,可能会以商业规模开发所选菌株。

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