Adenovirus Proteins II. N-Terminal Amino Acid Analysis

William G. Laver; J. Robert Suriano; Maurice Green

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Adenovirus Proteins II. N-Terminal Amino Acid Analysis

机译：腺病毒蛋白II。 N-末端氨基酸分析

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Adenovirus types 2, 4, 5, 6, 18, 21 and 27, were analyzed for N-terminal amino acids by use of 35S-labeled phenylisothiocyanate of high specific radioactivity. Two free N-terminal amino acids, alanine and glycine, were found in these viruses in the molar ratio (alanine-glycine) of 2.5:1, with the exception of type 2 where the ratio was 3.6:1 and type 5 where the ratio was 5.5:1 Adenovirus type 2 was disrupted by acetone treatment, and two protein fractions were obtained after sucrose gradient centrifugation. One of these fractions, which was associated with the viral deoxyribonucleic acid and comprised approximately 18% of the total protein of the virus, was greatly enriched with respect to N-terminal alanine and glycine.

机译：通过使用高特异性放射性的 35℃标记的苯二硫氰酸酯分析腺病毒类型2,4,5,6,18,21和27，对N-末端氨基酸分析。在这些病毒中发现了两个自由N-末端氨基酸，丙氨酸和甘氨酸，其摩尔比（丙氨酸 - 甘氨酸）为2.5：1，其中2型除以3.6：1，型比率是5.5：1腺病毒2型被丙酮处理中断，并且在蔗糖梯度离心后得到两种蛋白质级分。这些级分之一与病毒脱氧核糖核酸相关并包含约18％的病毒总蛋白质，相对于N-末端丙氨酸和甘氨酸大大富集。

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《Journal of Virology》 |1967年第4期|共6页
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William G. Laver; J. Robert Suriano; Maurice Green;
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1. Adenovirus Proteins II. N-Terminal Amino Acid Analysis [J] . William G. Laver, J. Robert Suriano, Maurice Green Journal of Virology . 1967,第4期

机译：腺病毒蛋白II。 N-末端氨基酸分析
2. Adenovirus Proteins II. N-Terminal Amino Acid Analysis [J] . William G. Laver, J. Robert Suriano, Maurice Green Journal of Virology . 1967,第4期

机译：腺病毒蛋白II。 N-末端氨基酸分析
3. Studies on the determination of the sequence of amino acids in peptides and proteins. 3. The synthesis of 3-(4′-dimethylamino-3′:5′-dinitrophenyl)hydantoin derivatives of various amino acids, and their use for the determination of N-terminal amino acids [J] . G. G. Evans, W. S. Reith The biochemical journal . 1954,第1期

机译：确定肽和蛋白质中氨基酸序列的研究。 3.各种氨基酸的3-（4'-二甲基氨基-3'：5'-二硝基苯基）乙内酰脲衍生物的合成及其在测定N端氨基酸中的用途
4. The Recognition of Subcellular Localization of Proteins Based on their N-terminal Amino Acid Sequence [C] . Jia Yun, Zhao Judong, Lu Jun The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议）论文集 . 2008

机译：基于蛋白质N末端氨基酸序列的蛋白质亚细胞定位识别
5. Structure-based design of mutant proteins: I. Molecular docking studies of amino acid binding to wild-type aminoacyl-tRNA synthetases. II. Structure-based design of mutant aminoacyl-tRNA synthetases for non-natural amino acid incorporation. [D] . Zhang, Deqiang. 2003

机译：基于结构的突变蛋白设计：I.氨基酸与野生型氨酰基tRNA合成酶结合的分子对接研究。二。非天然氨基酸掺入的突变型氨酰基-tRNA合成酶的基于结构的设计。
6. Adenovirus Proteins II. N-Terminal Amino Acid Analysis [O] . William G. Laver, J. Robert Suriano, Maurice Green 1967

机译：腺病毒蛋白II。 N端氨基酸分析
7. N-Terminal Amino Acid Sequence Determination of Proteins by N-Terminal Dimethyl Labeling: Pitfalls and Advantages When Compared with Edman Degradation Sequence Analysis [O] . Elizabeth Chang, Sergei Pourmal, Chun Zhou, 2016

机译：N-末端氨基酸序列通过N-末端二甲基标记的蛋白质测定：与Edman降解序列分析相比时的缺陷和优点

Adenovirus Proteins II. N-Terminal Amino Acid Analysis

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