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首页> 外文期刊>Journal of Virology >Amber Mutants of Bacteriophages T3 and T7 Defective in Phage-directed Deoxyribonucleic Acid Synthesis
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Amber Mutants of Bacteriophages T3 and T7 Defective in Phage-directed Deoxyribonucleic Acid Synthesis

机译：噬菌体T3和T7的琥珀色突变体在噬菌体指导的脱氧核糖核酸合成中有缺陷

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Amber mutants of the related phages T3 and T7 were isolated and tested for their ability to restore—as the wild type does—thymidine incorporation in ultraviolet (UV)-irradiated, UV-sensitive, nonpermissive host bacteria (Escherichia coli Bs-1). Most amber mutants had this ability. However, in both T3 and T7, mutants unable to promote thymidine incorporation under these conditions were found and classified into two well-defined complementation groups: T3DO-A and T3DO-B, T7DO-A and T7DO-B. Infection of Bs-1 cells with representatives of groups DO-A had the following characteristics: (i) phage-directed uridine uptake in UV-irradiated cells was reduced to less than 20% of normal; (ii) breakdown of host deoxyribonucleic acid (DNA) was delayed and incomplete; (iii) no serum-blocking antigens appeared; (iv) no cell lysis occurred; (v) the ability to exclude the heterologous wild type was impaired. Amber mutants of the DO-B groups, infecting Bs-1, were able to: (i) promote an efficient phage-directed uridine uptake in UV-irradiated cells; (ii) bring about rapid breakdown of host DNA; (iii) synthesize serum-blocking antigens; (iv) lyse the host cells, generally after the normal latent period; (v) exclude efficiently the heterologous wild type. Although physiological similarities between the respective DO-A mutants or DO-B mutants of T3 and T7 were evident, no physiological cross-complementation occurred, and genetic crosses gave no evidence of genetic homologies between groups of T3 and T7.
机译：分离出相关噬菌体T3和T7的琥珀色突变体，并测试其恢复的能力 - 随着野生型的紫外线（UV）r次抑制，紫外线敏感，无催化宿主细菌（大肠杆菌 B s-1 ）。大多数琥珀色突变体具有这种能力。然而，在T3和T7中，未发现在这些条件下促进胸苷掺入的突变体并分为两个明确定义的互补基团：T3DO-A和T3DO-B，T7DO-A和T7DO-B。 B S-1 细胞的感染具有群体的代表 - a具有以下特征：（i）紫外线辐照细胞中的噬菌体定向尿苷摄取降低至不到20％的正常; （ii）宿主脱氧核糖核酸（DNA）的分解延迟和不完全; （iii）没有出现血清阻断抗原; （iv）未发生细胞裂解; （v）排除异源野生型的能力受到损害。 DO-B组的琥珀色突变体，感染B S-1 ，可以：（i）促进UV辐照细胞中有效的噬菌体定向尿苷摄取; （ii）带来宿主DNA的快速分解; （iii）合成血清阻断抗原; （iv）含有宿主细胞，通常在正常潜在的时间内; （v）有效排除异源野生型。尽管T3和T7的各自的突变体或DO-B突变体之间的生理相似性是明显的，但没有发生生理交叉互补，并且遗传交叉没有在T3和T7的组之间没有遗传同源性的证据。

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