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首页> 外文期刊>Journal of Virology >Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome
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Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome

机译:NondefectiveAdenovirus 2-咪木病毒40杂交病毒的研究。猿猴病毒40移植抗原与早期病毒基因组特定区域的联系

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摘要

Two of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrids induce SV40 transplantation resistance in immunized hamsters. These two hybrids, Ad2+ND2 and Ad2+ND4, contain 32 and 43% of the SV40 genome, respectively. The pattern of induction of SV40 transplantation antigen (TSTA) by the various hybrids differentiates TSTA from both SV40 U and T antigens. Since the SV40 RNA induced by both these hybrids is early SV40 RNA, these findings confirm that TSTA is an early SV40 function. By combining available data on SV40 antigen induction by these hybrids with electron microscopy heteroduplex mapping studies, the DNA segment responsible for the induction of SV40 TSTA can be inferred to lie in the region between 0.17 and 0.43 SV40 units from the site on the SV40 chromosome cleaved by E. coli R1 restriction endonuclease.
机译:五种NondFective腺病毒2(AD2) - 综合病毒40(SV40)杂种中的两种诱导免疫仓鼠中的SV40移植抗性。这两个杂种AD2 + nd 2 和ad2 + nd 4 ,包含32和43%的sv40基因组分别。各种杂种的SV40移植抗原(TSTA)的诱导模式与SV40 U和T抗原分化了TSTA。由于两种杂种诱导的SV40 RNA是早期的SV40 RNA,因此这些发现证实TSTA是早期的SV40功能。通过将这些杂交种与电子显微镜异细胞杂交映射研究组合的可用数据,可以推断负责SV40 TSTA的诱导的DNA片段,以在SV40染色体上的斑点的位置处位于0.17和0.43 sv40单元之间的区域。通过 e。 COLI R 1 限制性内切核酸酶。

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