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首页> 外文期刊>Journal of Virology >Reovirus-induced Ribonucleic Acid Polymerase
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Reovirus-induced Ribonucleic Acid Polymerase

机译：reovirus诱导的核糖核酸聚合酶

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A virus-induced ribonucleic acid (RNA) polymerase activity was found in L cells infected with type 3 reovirus. Most of the enzyme is associated with the “large particle” fraction of the infected cells. The enzyme first appeared at 3 to 5 hr after infection and increased in amount until 7 to 9 hr. All four ribonucleoside triphosphates are incorporated in vitro into an acid-insoluble form by the enzyme. The major part of the product formed in vitro is a double-stranded RNA indistinguishable from viral RNA by electrophoresis on polyacrylamide gel. Approximately 40% of the product is a single-stranded RNA of relatively small molecular weight. More than 95% of the nucleotides incorporated into double-stranded RNA by the enzyme are bound in internal 3′-5′-phosphodiester linkages extending back from both 3′- and 5′-termini of the RNA strands.
机译：在感染3型reovirus的L细胞中发现病毒诱导的核糖核酸（RNA）聚合酶活性。大多数酶与受感染细胞的“大颗粒”分数相关联。酶首先在感染后3至5小时出现，升高至7至9小时。所有四种核糖核苷三磷酸盐通过酶在体外掺入酸不溶性形式中。在体外形成的产品的主要部分是通过聚丙烯酰胺凝胶上的电泳从病毒RNA无法区分的双链RNA。大约40％的产物是分子量相对小的单链RNA。酶中掺入双链RNA中的95％以上的核苷酸在内部3'-5'-磷酸二酯键中结合，从3'-和5'-末端的RNA链延伸。

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