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首页> 外文期刊>Journal of bacteriology >Isolation of specialized transducing bacteriophages carrying deletions of the regulatory region of the Escherichia coli K-12 tryptophan operon.
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Isolation of specialized transducing bacteriophages carrying deletions of the regulatory region of the Escherichia coli K-12 tryptophan operon.

机译:分离专业转换噬菌体缺少大肠杆菌K-12色氨酸操纵子监管区的缺失。

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A lysogen of a wild-type strain of Escherichia coli K-12 carrying a heat-inducible lambda-phi80 hybrid prophage was induced to yield transducing phages carrying all of the structural genes of the tryptophan operon. The presence or absence of elements of the trp regulatory region was determined by examining the effects of lambda genes N and cI on trp gene expression. The phages were further characterized by transduction studies and by examining anthranilate synthetase (EC 4.1.3.27) (TRYPE+D) synthesis in the presence of the lambda cI product. A number of phages deleted for the trp promoter were found. Recombination studies between trpOc bacteria and the transducing phages have yielded information that can be used to order the trp end points of some phages and to provide an estimate of the size of the trp promoter region.
机译:诱导携带热诱导λ-Phi80杂交血管预先杂交出版物的野生型大肠杆菌K-12的溶菌性,得到携带色氨酸操纵子的所有结构基因的转换噬菌体。通过检查λ基因N和CI对TRP基因表达的影响来确定TRP调节区元素的存在或不存在。通过转导研究和通过在λCI产物的存在下检查邻苯酸盐合成酶(EC 4.1.3.27)(Thype + D)合成,进一步表征了噬菌体。发现了用于TRP启动子的许多噬菌体。 TRPOC细菌和转导噬菌体之间的重组研究产生的信息可用于订购一些噬菌体的TRP终点并提供TRP启动子区域的尺寸的估计。

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