Membranes were isolated and purified from nutrient broth-yeast extract- and hexadecane-grown cells of Acinetobacter sp. strain HO1-N. Two membrane fractions were isolated from nutrient broth-yeast extract-grown cells, the cytoplasmic membrane and the outer membrane. In addition to these two membrane fractions, a unique membrane fraction was isolated from hexadecane-grown cells (band 1) and characterized as a lipid-rich, low-density membrane containing high concentrations of hexadecane. The outer membrane preparations of Acinetobacter, obtained from nutrient broth-yeast extract- and hexadecane-grown cells, exhibited a low ratio of lipid phosphorus to protein and contained phospholipase activity and 2-keto-3-deoxyoctulosonic acid. Phosphatidic acid cytidyltransferase, adenosine triphosphatase, and reduced nicotinamide adenine dinucleotide oxidase were recovered almost exclusively in the cytoplasmic membrane fractions. The cytoplasmic membrane fractions contained 20 to 25 polypeptide species on sodium dodecyl sulfate-polyacrylamide gels, and the outer membrane fractions contained 15 to 20 polypeptide species. A major polypeptide species with an apparent molecular weight of approximately 42,000 to 44,000 was found for all outer membrane fractions. The buoyant densities of the cytoplasmic membrane fractions and the outer membrane fractions were closely similar, necessitating their separation by differential centrifugation. Band 1 of hexadecane-grown cells had a ratio of lipid phosphorus to protein that was almost twice that of cytoplasmic membrane and a correspondingly low buoyant density (1.086 g/cm3). Enzyme activities associated with band 1 were identical to those associated with the cytoplasmic membrane. The electrophoretic banding pattern of band 1 was essentially identical to the banding pattern of the cytoplasmic membrane. The phospholipid and neutral lipid compositions of the isolated membrane fractions were determined as qualitatively similar, with significant quantitative differences. The ultrastructure characteristics of the respective membrane fractions were examined by the negative-stain technique.
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机译：分离膜，并从营养肉汤酵母提取物和十六烷生长细胞的分离和纯化的分枝杆菌。菌株HO1-N.从营养肉汤酵母提取物生长细胞，细胞质膜和外膜分离出两个膜级分。除了这两个膜级分之外，独特的膜级分是从十六烷种植的细胞（带1）中分离的，并表征为含有高浓度的十六甲烷的富含脂质的低密度膜。从营养肉汤 - 酵母提取物和十六烷生长的细胞获得的外膜制剂表现出脂质磷与蛋白质的低比例，并含有磷脂酶活性和2-酮-3-脱氧核酸酸。在细胞质膜级分几乎完全回收磷脂酸细胞转移酶，腺苷三磷酸酶和还原的烟酰胺腺嘌呤二核苷酸氧化酶。细胞质膜馏分在十二烷基硫酸钠 - 聚丙烯酰胺凝胶上含有20至25种多肽物质，外膜级分含有15至20个多肽物种。对于所有外膜级分，发现具有表观分子量的主要多肽物种约42,000至44,000。细胞质膜级分和外膜级分的浮力密度密切相似，需要通过差动离心分离。十六烷生长的细胞的带1的脂质磷与蛋白质的比例几乎是细胞质膜的两倍和相应的低浮力密度（1.086g / cm 3）。与带1相关的酶活性与与细胞质膜相关的酶活性相同。带1的电泳条带图案与细胞质膜的配音图案基本相同。将分离的膜级分的磷脂和中性脂质组合物定义相似，具有显着的定量差异。通过阴性染色技术检查各膜级分的超微结构特性。
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