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首页> 外文期刊>Journal of Virology >Predominant immediate-early transcripts of human cytomegalovirus AD 169.
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Predominant immediate-early transcripts of human cytomegalovirus AD 169.

机译：主要的立即早期的人巨细胞病毒AD 169。

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摘要

Transcription of the human cytomegalovirus genome (strain AD 169) was investigated at the immediate-early (IE) time after infection, by using cycloheximide to suppress virus-specific protein synthesis. In total cell RNA, four predominant IE transcripts were found which were encoded by one contiguous region of the long unique segment between map units 0.06 and 0.16 in prototype arrangement of human cytomegalovirus AD 169 DNA. Analysis by Northern blot hybridizations demonstrated that the transcripts possessed a size of 1.9, 2.2, 2.3, and 5.0 kilobases, respectively. Coding sequences and directions of transcriptions were mapped by Northern blots and hybridizations with oligodeoxythymidylic acid-primed and randomly primed cDNA. The 1.9-, 2.2-, and 2.3-kilobase RNAs were found in the polyadenylated fraction of IE RNA exclusively; in contrast, a part of the 5.0-kilobase RNA appeared polyadenylated, although the majority of the same transcript was found in the nonpolyadenylated pool. Also, different from the other IE genes, the DNA coding for the 5.0-kilobase IE RNA was transcribed in high quantities during the late phase of virus replication, suggesting an exemption from the temporal regulation of herpesvirus transcription.
机译：通过使用环己酰亚胺抑制病毒特异性蛋白质合成，在感染后早期（IE）时间来研究人巨细胞病毒基因组（菌株AD 169）的转录。在总细胞RNA中，发现四个主要的IE转录物，其在人巨细胞病毒AD 169 DNA的原型布置中由MAP单元0.06和0.16之间的长唯一区段的一个连续区域编码。 Northern印迹杂交的分析表明，转录物分别具有1.9,2.2,2.3和5.0千碱基的尺寸。通过Northern印迹和随机灌注的cDNA的Northern印迹和杂交的编码序列和转录的编程和杂交。在IE RNA的多腺苷酸化分数中发现1.9-，2.2-和2.3千碱基RNA;相反，5.0千碱酶RNA的一部分出现了多腺苷酸化，尽管在非聚淀粉化的池中发现了大部分相同的转录物。此外，与其他IE基因不同，在病毒复制的晚期期间，编码5.0千碱基IE RNA的DNA在病毒复制的后期转录，表明患有HERPESVIRUS转录的时间调节的豁免。

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