• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nucleic acids research >Isolation and characterization of endonuclease J: a sequence-specific endonuclease cleaving immunoglobulin genes
【24h】

Isolation and characterization of endonuclease J: a sequence-specific endonuclease cleaving immunoglobulin genes

机译：内切核酸酶J：序列特异性内切核酸酶切割免疫球蛋白基因的分离与表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An endonuclease activity which cleaves close to the recombination sites of the imnunoglobulin JK segments was found in extracts of chicken bursa of Fabricius and characterized after partial purification. The enzyme preparation also cleaved a VK segment at its 3′ end. A similar activity was found in mouse liver, mouse myelomas and Hela cells. The enzyme designated as endonuclease J introduces double-stranded cleavages preferentially at sequences containing G clusters of pBR322 as well as the JK segments. However, not all the G clusters were cleaved by endonuclease J, suggesting that the enzyme recognizes additional sequences. Deletion of the conserved nonamer (GGTTTTTGT) located immediately 5′ to the JK4 segment drastically reduced the cleavage activity of its immediate downstream G cluster. Although biological function of endonuclease J is not clear at this stage, the possibilities of its involvement in the immunoglobulin gene recombination and general recombination were discussed.
机译：内切核酸酶活性，其靠近ImnunogloblobulinJ K / sub>段的重组位点被发现在Fabryius的鸡囊提取物中，并且在部分纯化后表征。酶制剂也在其3'末端切割v k / sub>段。在小鼠肝脏，小鼠髓鞘和HeLa细胞中发现了类似的活性。指定为内切核酸酶J的酶首先在含有PBR322的G簇的序列以及J k 段的序列中引入双链裂解。然而，并非所有G簇都是通过内切核酸酶J裂解的，表明酶识别额外的序列。删除位于J K4 段的水平5'位于J K4 段的保守的非凡（GGTTTTGT）大大降低了其立即下游G集群的切割活动。尽管在该阶段，核酸核酸酶J的生物学功能尚不清楚，但讨论了其参与免疫球蛋白基因重组和一般重组的可能性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号