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首页> 外文期刊>Infection and immunity >Invasion of HEp-2 cells by fecal isolates of Aeromonas hydrophila.
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Invasion of HEp-2 cells by fecal isolates of Aeromonas hydrophila.

机译：患有Aeromonas疏水层的粪便分离株入侵Hep-2细胞。

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Human diarrheal isolates of Aeromonas hydrophila were found to be invasive in HEp-2 cell monolayers. Microscopic examination of two isolates from patients with symptoms of dysentery revealed that infected HEp-2 cells contained up to 50 bacteria localized within areas of the cytoplasm. A quantitative invasion assay was developed, using viable counts of total intracellular bacteria. Five fecal isolates of A. hydrophila were classified as invasive, with an average of 0.1 up to 2 bacteria per HEp-2 cell, as compared to 7 for an invasive Escherichia coli strain. 3 other fecal isolates, 1 reference strain, and 10 water isolates of A. hydrophila were similar to a noninvasive E. coli strain, with less than 0.008 bacteria per HEp-2 cell. All isolates were screened for plasmid DNA; no common plasmid was found in the invasive strains, and the loss of a 6.0-megadalton plasmid from one of these strains had no observable effect on invasiveness. Thus some A. hydrophila isolates are capable of cellular penetration and replication, and this may be an important chromosomally determined virulence property of the organism.
机译：发现Healonas疏水物的人腹泻分离物在Hep-2细胞单层中被侵入性。对痢疾症状患者的两分离株的显微镜检查显示，感染的HEP-2细胞含有多达50个细菌在细胞质区域内的细菌。开发了使用总细胞内细菌的可行计数的定量侵袭测定。与侵入性大肠杆菌菌株的7相比，疏水层的五个粪便分离为侵入性，平均每HEP-2细胞均为0.1至2个细菌。 3其他粪便分离物，1个参比菌株和10个水分分离物与非侵入式大肠杆菌菌株相似，每HEP-2细胞小于0.008细菌。筛选所有分离株的质粒DNA;在侵入菌株中没有发现常见质粒，并且来自这些菌株中的一个6.0megadalton质粒的损失对侵袭性没有可观察的影响。因此，一些A.疏水层分离物能够进行细胞渗透和复制，这可能是生物体的重要染色体确定的毒力特性。

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