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首页> 外文期刊>Journal of bacteriology >Inactivation of prophage in ultraviolet-irradiated Escherichia coli: dependence on recA gene activity.
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Inactivation of prophage in ultraviolet-irradiated Escherichia coli: dependence on recA gene activity.

机译：紫外线辐照大肠杆菌灭活的灭活：依赖性研究RECA基因活性。

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The fate of the prophage part of the lysogenic chromosome was followed in the course of post-ultraviolet incubation. For this purpose, lambda cI857 ind prophage, which can be induced by heat but not by ultraviolet light, was used. The prophage, intially more resistant than its repair-proficient host cell, was rapidly inactivated. This inactivation was not caused by the impaired capacity of irradiated cells to support growth of the phage. Over the entire dose range tested, little, if any, sensitivity difference between the host and the prophage was found at the end of cell division delay. Rapid inactivation of the prophage was also observed in uvr cells after small doses of ultraviolet light. The same small doses did not cause inactivation in lysogens carrying a mutation in the gene recA. This suggests that the functional gene recA is required for inactivation of the prophage part of the lysogenic chromosome.
机译：在后紫外线孵育过程中，遵循了溶血物染色体的丙虫部分的命运。为此目的，使用λCI857Ind Premhage，其可以通过热而不是紫外线诱导。迅速灭活，突出，抗预测性宿主细胞具有柔软的抗性。这种灭活不是由辐照细胞占噬菌体生长的损害。在细胞分割延迟结束时发现了宿主与前孔之间的敏感性差异的少量剂量范围。在小剂量的紫外线后，在UVR细胞中也观察到出版物的快速失活。相同的小剂量没有导致在基因reca中携带突变的溶液中灭活。这表明官能基因reca是灭活溶血物染色体的灭活。

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