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首页> 外文期刊>Journal of bacteriology >recE4-Independent recombination between homologous deoxyribonucleic acid segments of Bacillus subtilis plasmids.
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recE4-Independent recombination between homologous deoxyribonucleic acid segments of Bacillus subtilis plasmids.

机译:在枯草芽孢杆菌质粒的同源脱氧核​​糖核酸区段之间接收4-Indumity重组。

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摘要

A plasmid (pLS104) carrying a tandem repetition of the leu region of the Bacillus subtilis chromosome arose spontaneously from pLS103, which carried a single copy of the leu region. Plasmid preparations from strains harboring pLS104 also contained the original plasmid, pLS103, and, in some preparations, plasmids carrying three or four repetitions of the leu region. These plasmids were shown to be generated by recombination between homologous deoxyribonucleic acid (DNA) segments in the tandemly repeated DNA regions on the plasmids, but not by recombinations between specific DNA sites. These phenomena were observed in a recE4-Independent background, showing that recombination of the homologous DNA sequences does not require the recE-Independent gene product(s).
机译:携带枯草芽孢杆菌染色体的Leu区域的串联重复的质粒(PLS104)从PLS103自发地产生,该PLS103携带单一的Leu区域。来自宿株PLS104的菌株的质粒制剂还含有原有的质粒,PLS103,以及在一些制剂中,具有三个或四次重复的Leu区域的质粒。显示这些质粒通过在质粒上的串联重复的DNA区域中的同源脱氧核​​糖核酸(DNA)区段之间的重组产生,但不是通过特定DNA位点之间的重组。在RECED4无关的背景中观察到这些现象,表明同源DNA序列的重组不需要受托无关的基因产物。

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