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首页> 外文期刊>Infection and immunity >Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosus.
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Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosus.

机译：链球菌和非链球菌口腔细菌的常见抗原：来自链球菌，链球菌，乳酸杆菌，枸杞子和放样粘液的细胞外和细胞壁相关抗原的免疫化学研究。

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Soluble extracellular antigens (ESA) were prepared from the culture supernatant of exponential growing cells of Streptococcus sanguis OMZ 9 by a combination of ammonium sulfate precipitation and chromatography on a Bio-Gel P6 column. Soluble cell wall antigens (WEA) were obtained from the bacterial pellet by extraction with 1 M phosphate buffer (pH 6). Antisera against whole cells of S. sanguis and S. mutans of different serotypes, 10% trichloroacetic extracts of bacterial cell walls, dextran, ESA, and WEA were prepared by injecting the different antigens several times in rabbits. ESA and WEA were prepared from a representative strain of Bratthall's seven serological groups, Lactobacillus salivarius, and Actinomyces viscosus. All sera showed various agglutinin titers against heat-killed cells, and titers were generally higher with homologous cells. The comparison of the different antigens using agar gel diffusion and immunoelectrophoresis showed the presence of extracellular common antigens in both ESA and WEA between the different strains. Absorption of anti-ESA sera with WEA, and anti-WEA sera with ESA, showed the existence of a specific antigen common to all bacteria in each fraction. Enzymatic treatment of the antigen before immunodiffusion demonstrated the protein nature of the two antigens present in ESA and WEA.
机译：通过硫酸铵沉淀和生物凝胶P6塔的氨基硫酸铵沉淀和色谱法，由链球菌血管综合体OMZ 9的指数生长细胞的培养上清液制备可溶性细胞外抗原（ESA）。通过用1M磷酸盐缓冲液（pH6）萃取从细菌颗粒获得可溶性细胞壁抗原（WEA）。通过在兔子中注射不同的抗原，在兔中注入不同的抗原，制备抗血清型的全细胞和不同血清型的全细胞的抗血清，其细菌细胞壁，葡聚糖，esa和wea的抗血清酸纤维素。 ESA和WEA是由布拉特七血清群，乳酸杆菌唾液和放样粘液的代表性菌株制备的。所有血清显示各种氨基蛋白滴度抵抗热杀死的细胞，并且滴度通常用同源细胞更高。使用琼脂凝胶扩散和免疫电泳的不同抗原的比较显示出在不同菌株之间的ESA和WEA中存在细胞外常见抗原。用WEA和抗WEA血清吸收抗ESA血清，患有ESA的抗WEA血清，表明每个级分中所有细菌共用的特定抗原存在。免疫分离前的抗原酶处理证明了ESA和WEA中存在的两种抗原的蛋白质。

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