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首页> 外文期刊>Journal of Clinical Microbiology >Controlled evaluation of the agar-slide and radiometric blood culture systems for the detection of bacteremia and fungemia.
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Controlled evaluation of the agar-slide and radiometric blood culture systems for the detection of bacteremia and fungemia.

机译:对琼脂 - 载玻片和辐射血液培养系统进行控制评价,用于检测菌血症和血液血症。

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A commercially available agar-slide blood culture bottle (Septi-Chek; Roche Diagnostics, Div. Hoffman-La Roche, Inc., Nutley, N.J.) was compared with the radiometric blood culture system (BACTEC; Johnston Laboratories, Inc., Towson, Md.) in 8,544 paired blood cultures from adult patients. The systems were inoculated with equal volumes (10 ml) of blood. Overall, there was no statistically significant difference between the two systems in the recovery of clinically important microorganisms, but significantly more members of the family Enterobacteriaceae other than Escherichia coli were detected by the agar-slide system (P less than 0.005). The agar-slide system detected more fungi, and the BACTEC detected more anaerobic bacteria; however, small numbers of recovered organisms precluded statistical significance. When microorganisms grew in both systems, their presence was detected one or more days earlier in the BACTEC (P less than 0.001). More contaminants grew in the agar-slide system (P less than 0.001). Both systems performed well, and either system should provide high yield and prompt detection of positive blood cultures in patients with bacteremia and fungemia if used in an optimal way as recommended by the respective manufacturers.
机译:市售的琼脂血液培养瓶(SEPTI-CHEK;罗氏诊断,DIV。Hoffman-La Roche,Inc.,Nuffman-La Roche,Inc.)与辐射血液培养系统(Bactec; Johnston Laboratories,Inc.,Towson,Towson, MD。)来自成年患者的8,544份血液培养物。该系统被同等体积(10mL)血液接种。总体而言,两种系统在临床上重要的微生物中恢复没有统计学意义,但由于琼脂 - 滑动系统检测到除大肠杆菌外的家庭肠杆菌的成员显着增加(P小于0.005)。琼脂滑动系统检测到更多真菌,并且Bactec检测到更多的厌氧细菌;然而,少量回收的生物体排除统计学意义。当微生物在两个系统中增长时,在Bactec中检测到它们的存在一或更长时间(P小于0.001)。更多的污染物在琼脂滑动系统中增长(P小于0.001)。两种系统表现良好,如果以相应制造商推荐的方式使用,则两种系统应提供高产率和迅速检测患有菌血症和血淋力的患者的阳性血液培养。

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