Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene.

D Colau; I Negrutiu; M Van Montagu; J P Hernalsteens

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Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene.

机译：农杆菌肿瘤癌酿酒酵母癌症患者后苏氨酸脱水酶缺乏烟草型突变体的辅酶介导的酿酒酵母。

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The Saccharomyces cerevisiae ILV1 gene, encoding threonine dehydratase (EC 4.2.1.16) was fused to the transferred DNA nopaline synthase promoter and the 3' noncoding region of the octopine synthase gene. It was introduced, by Agrobacterium tumefaciens-mediated gene transfer, into an isoleucine-requiring Nicotiana plumbaginifolia auxotroph deficient in threonine dehydratase. Functional complementation by the ILV1 gene product was demonstrated by the selection of several transformed lines on a medium without isoleucine and by the identification in these lines of the yeast threonine dehydratase activity. This is the first example illustrating the complementation of a plant auxotroph by transfection with a cloned gene.

机译：将苏氨酸脱水酶（EC 4.2.1.16）编码的酿酒酵母ILV1基因融合给章鱼合成酶基因的转移的DNA Nopaline合成酶启动子和3'非编码区。通过Tumefaciens介导的基因转移介绍，介绍了一种在苏氨酸脱水酶中的异亮氨酸尼古尼氏植物肺炎肺炎缺乏症。通过在没有异亮氨酸的培养基上选择几条转化的线和通过在这些酵母苏氨酸脱水酶活性的培养基中鉴定来证明ILV1基因产物的功能互补。这是第一个实施例，其通过用克隆基因转染植物滋养营养症的互补。

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《Molecular and Cellular Biology》 |1987年第7期|共6页
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D Colau; I Negrutiu; M Van Montagu; J P Hernalsteens;
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1. Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene. [J] . D Colau, I Negrutiu, M Van Montagu, Molecular and Cellular Biology . 1987,第7期

机译：农杆菌肿瘤癌酿酒酵母癌症患者后苏氨酸脱水酶缺乏烟草型突变体的辅酶介导的酿酒酵母。
2. Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene. [J] . D Colau, I Negrutiu, M Van Montagu, Molecular and Cellular Biology . 1987,第7期

机译：农杆菌肿瘤癌酿酒酵母癌症患者后苏氨酸脱水酶缺乏烟草型突变体的辅酶介导的酿酒酵母。
3. A cdc-like autolytic Saccharomyces cerevisiae mutant altered in budding site selection is complemented by SPO12, a sporulation gene. [J] . G Molero, M Yuste-Rojas, A Montesi, Journal of bacteriology . 1993,第20期

机译：在发芽位点选择中改变的类似cdc的自溶啤酒酵母突变体由孢子形成基因SPO12补充。
4. Overexpression of alpha-D-mannose-1-phosphate guanyl-transferase encoding ggene restores the viability of the saccharomyces cerevisiae mutants affected in early steps of glycoconjugate formation [C] . Annal Janik, Joanna Kruszewska, Urszula Lenart, Carbohydrate bioengineering meeting . 1999

机译：编码ggene的α-D-甘露糖-1-磷酸鸟苷转移酶的过表达恢复了在糖缀合物形成早期受到影响的酿酒酵母突变体的活力
5. Identification of new pathogenicity genes in Magnaporthe oryzae through the construction of an Agrobacterium tumefaciens-mediated insertion mutant library. [D] . Betts, Melania Figueroa. 2007

机译：通过根癌农杆菌介导的插入突变体文库的构建，鉴定稻米中新的致病基因。
6. Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene. [O] . D Colau, I Negrutiu, M Van Montagu, 1987

机译：根癌农杆菌介导的酿酒酵母ILV1基因转移后苏氨酸脱水酶缺陷型烟草李青烟草突变体的补充。
7. Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene. [O] . Colau, D, Negrutiu, I, Van Montagu, M, 1987

机译：根癌农杆菌介导的啤酒酵母ILV1基因转移后，苏氨酸脱水酶缺陷型烟草李子烟草突变体的互补。

Complementation of a threonine dehydratase-deficient Nicotiana plumbaginifolia mutant after Agrobacterium tumefaciens-mediated transfer of the Saccharomyces cerevisiae ILV1 gene.

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