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首页> 外文期刊>Journal of Virology >pp60c-src has less affinity for the detergent-insoluble cellular matrix than do pp60v-src and other viral protein-tyrosine kinases.
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pp60c-src has less affinity for the detergent-insoluble cellular matrix than do pp60v-src and other viral protein-tyrosine kinases.

机译：PP60C-SRC对洗涤剂不溶性细胞基质的亲和力较低，而不是PP60V-SRC和其他病毒蛋白 - 酪氨酸激酶。

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摘要

A difference in affinity for a Nonidet P-40-insoluble cellular matrix was observed between the products of the viral and cellular src genes. It has previously been demonstrated that pp60v-src is associated with a detergent-insoluble matrix containing the cellular cytoskeleton (J. G. Burr, G. Dreyfuss, S. Penman, and J. M. Buchanan, Proc. Natl. Acad. Sci. USA 77:3484-3488, 1980). We observed a similar association of the transforming proteins of Fujinami sarcoma virus (P130gag-fps) and Yamaguchi 73 avian sarcoma virus (P90gag-yes), both of which are tyrosine-specific protein kinases. However, we found that the endogenous c-src product, pp60c-src, was not tightly bound to the detergent-insoluble matrix. This does not appear to have been due to differences in the cytoskeleton between transformed and nontransformed cells since pp60c-src was also solubilized by nonionic detergent in cells transformed by Rous sarcoma virus. This difference in the affinities of the v-src and c-src products for cytoskeletal proteins may contribute to the inability of pp60c-src to transform cells.
机译：在病毒和细胞SRC基因的产物之间观察到非身分P-40-不溶性细胞基质的亲和力的差异。先前已经证明，PP60V-SRC与含有细胞细胞骨架的洗涤剂不溶性基质有关（JG Burr，G. Dreyfuss，S. Benman和JM Buchanan，Proc。Natl。Acad。SCI。美国77：3484- 3488,980）。我们观察了富士胺肉瘤病毒（P130GAG-FPS）和Yamaguchi 73禽Sarcoma病毒（P90GAG-YES）的转化蛋白质的类似关联，这两种是酪氨酸特异性蛋白激酶。然而，我们发现内源性C-SRC产物PP60C-SRC未与洗涤剂不溶性基质紧密结合。由于PP60C-SRC在由Rous Sarcoma病毒转化的细胞中的非离子洗涤剂中也溶解，这似乎没有是由于转化和非转化细胞之间的细胞骨架的差异。用于细胞骨架蛋白的V-SRC和C-SRC产品的亲和力的这种差异可能导致PP60C-SRC无法转化细胞。

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