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首页> 外文期刊>Journal of Virology >Enhancers and trans-acting E2 transcriptional factors of papillomaviruses.
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Enhancers and trans-acting E2 transcriptional factors of papillomaviruses.

机译：增强剂和转作乳头瘤病毒的转录因子。

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摘要

The upstream regulatory regions of human papillomavirus (HPV) types 1, 6b, 7, 11, 16, and 18, bovine papillomavirus type 1, and cottontail rabbit papillomavirus were cloned into transcriptional enhancer assay plasmids which carry the simian virus 40 early promoter lacking its own enhancer and the bacterial gene encoding chloramphenicol acetyltransferase (EC 2.3.1.28) (CAT). Enhancer activity, reflected by CAT gene expression, was detected in all of the upstream regulatory regions tested only when the recombinants were cotransfected with plasmids which express an intact E2 open reading frame of HPV types 1 and 11 or bovine papillomavirus type 1. Each E2 protein stimulated the enhancer from the same virus and, to somewhat lesser degrees, also those from the heterologous viruses. Hence, the enhancer and the E2 protein are functionally conserved among papillomaviruses. There was some nonreciprocity in the extent of trans-activation in heterologous E2-enhancer interactions. Primer extension analyses demonstrated that the E2 proteins increased the abundance of CAT gene mRNA. Tandem multiplication of the HPV type 11 enhancer sequence dramatically increased its response to E2 stimulation; this is possibly relevant to the pathogenicity of papillomaviruses.
机译：人乳头瘤病毒（HPV）类型1,6B，7,11,16和18型，牛乳头瘤病毒1，以及棉尾兔乳头瘤病毒的上游调节区被克隆到转录增强剂测定质粒中，携带Simian病毒40早期启动子缺乏其编码氯霉素乙酰转移酶（EC 2.3.1.28）（猫）的自己的增强剂和编码细菌基因。在所有上游调节区域中检测到猫基因表达的增强剂活性仅在通过表达HPV类型1和11种或牛乳头瘤病毒1.每种E2蛋白的完整E2开放读数框架的质粒中进行重组调节区域。每个E2蛋白从同一病毒中刺激增强剂，并稍微较小的程度，也是来自异源病毒的病毒。因此，增强剂和E2蛋白在乳头瘤病毒中具有功能挽救。异源E2-增强剂相互作用的晶体激活程度中存在一些非侵略性。引物延伸分析证明E2蛋白增加了猫基因mRNA的丰富。 HPV型11型增强子序列的繁殖倍增显着增加了对E2刺激的响应;这可能与乳头瘤病毒的致病性相关。

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