The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species.

V M Olkkonen; D H Bamford

首页> 外文期刊>Journal of Virology >The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species.

【24h】

The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species.

机译：含脂质的双链RNA噬菌体PHI 6的核衣壳含有由单个多肽物种组成的蛋白质骨架。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The nucleocapsid of the enveloped double-stranded RNA bacteriophage phi 6 was isolated by extraction with the nonionic detergent Triton X-114 and subjected to disruption analysis with chelating and protein-denaturing agents. The subnucleocapsid particles were separated in rate-zonal sucrose gradients, and their ultrastructure and protein composition were analyzed. The role of divalent cations in the nucleocapsid structure was studied by using a precipitation assay of the isolated nucleocapsid proteins. The phi 6 nucleocapsid had a cagelike skeleton consisting of a single polypeptide species (P1). Two other proteins (P2 and P4) were associated with the P1 cage. These three early proteins were previously known to be involved in the RNA synthesis machinery of the virus. The stability of the nucleocapsid surface lattice consisting of protein P8 was dependent on Ca2+ ions.

机译：通过用非离子洗涤剂Triton X-114提取包膜双链RNA噬菌体PHI 6的核衣壳，并用螯合和蛋白质 - 变性剂进行破坏分析。将亚核遗传粒颗粒以速率 - 左旋蔗糖梯度分开，分析其超微结构和蛋白质组合物。通过使用分离的核衣壳蛋白的沉淀测定来研究二价阳离子在核衣壳结构中的作用。 PHI 6 Nuclecapsid具有由单个多肽物种（P1）组成的CAGELIKE骨架。另外两种蛋白质（P2和P4）与P1笼相关。以前已知这三种早期蛋白质参与病毒的RNA合成机械。由蛋白质P8组成的核衣壳表面晶格的稳定性依赖于Ca2 +离子。

著录项

来源
《Journal of Virology》 |1987年第8期|共6页
作者
V M Olkkonen; D H Bamford;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species. [J] . V M Olkkonen, D H Bamford Journal of Virology . 1987,第8期

机译：含脂质的双链RNA噬菌体PHI 6的核衣壳含有由单个多肽物种组成的蛋白质骨架。
2. The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species. [J] . V M Olkkonen, D H Bamford Journal of Virology . 1987,第8期

机译：含脂质的双链RNA噬菌体PHI 6的核衣壳含有由单个多肽物种组成的蛋白质骨架。
3. IN VITRO PACKAGING OF THE SINGLE-STRANDED RNA GENOMIC PRECURSORS OF THE SEGMENTED DOUBLE-STRANDED RNA BACTERIOPHAGE PHI-6 - THE THREE SEGMENTS MODULATE EACH OTHERS PACKAGING EFFICIENCY [J] . Frilander M., Bamford DH. Journal of Molecular Biology . 1995,第3期

机译：分段双链RNA噬菌体PHI-6的单链RNA基因组学前体的体外包装-3个片段可调节其他每个包装的效率
4. Construction of RNA-binding proteins having nucleobase amino acids based on HIV-1 nucleocapsid protein [C] . Tsuyoshi Takahashi, Akihiko Ueno, Hisakazu Mihara the International Peptide Symposium . 2001

机译：基于HIV-1核衣壳蛋白的核碱基氨基酸的RNA结合蛋白的构建
5. SINGLE-STRANDED-DNA SP6 AND T7 BACTERIOPHAGE PROMOTER PLASMIDS FOR ENGINEERING MUTANT RNAS AND PROTEINS: EXTENSIONS AND DELETIONS AT THE AMINO TERMINUS WHICH ALTER THE ACTIVITY OF PREPROPARATHYROID HORMONE SIGNAL PEPTIDE. [D] . MEAD, DAVID ALAN. 1986

机译：用于工程突变RNA和蛋白质的单链DNA SP6和T7细菌噬菌体启动子质粒：在氨基末端的延伸和缺失，同时改变了前原甲状腺激素激素信号肽的活性。
6. The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species. [O] . V M Olkkonen, D H Bamford 1987

机译：含脂质的双链RNA噬菌体phi 6的核壳含有由单个多肽组成的蛋白质骨架。
7. The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species [O] . V M Olkkonen, D H Bamford 1987

机译：含脂质的双链RNA噬菌体PHI 6的核衣壳含有由单个多肽物种组成的蛋白质骨架

The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage phi 6 contains a protein skeleton consisting of a single polypeptide species.

摘要

著录项

相似文献

相关主题

期刊订阅