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首页> 外文期刊>Journal of Virology >Activated Ha-ras can cooperate with defective simian virus 40 in the transformation of nonestablished rat embryo fibroblasts.
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Activated Ha-ras can cooperate with defective simian virus 40 in the transformation of nonestablished rat embryo fibroblasts.

机译：活化的HA-Ras可以在非建质性的鼠胚成纤维细胞的转化中与钝猿病毒40配合。

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摘要

We studied the ability of activated Ha-ras to cooperate with simian virus 40 (SV40) in the transformation of nonestablished rat embryo fibroblasts. Cotransfection with Ha-ras greatly accelerated the rate of focus induction by wild-type SV40. Moreover, a series of transformation-defective SV40 mutants could be partially complemented by Ha-ras. This was true not only for mutants retaining an intact N-terminal immortalization-competent domain, but also for a nonkaryophilic SV40 mutant. In the latter case, all detectable T antigen was cytoplasmic, indicating that efficient transformation can be achieved through the interaction of two nonnuclear proteins. By employing cell lines derived with various SV40 mutants, it was determined that the ability to complex with p53 depends on the integrity of a relatively large region in the C-terminal half of large T. Finally, we report that nonkaryophilic SV40 large T forms a complex with the major heat shock protein HSP70, and we discuss its possible implications.
机译：我们研究了活化的HA-RA与Simian病毒40（SV40）的能力在非建筑的大鼠胚胎成纤维细胞的转化中。具有HA-RA的COTRANSFETET大大加速了野生型SV40的聚焦诱导速率。此外，一系列转化缺陷的SV40突变体可以通过HA-Ras部分互补。这不仅适用于保留完整的N-末端永生化型域的突变体，而且是对于非亚里亚替核酸的SV40突变体。在后一种情况下，所有可检测的T抗原是细胞质，表明通过两种非核素的相互作用可以实现有效的转化。通过使用与各种SV40突变体衍生的细胞系，确定与P53复合的能力取决于C末端大的T的相对大区域的完整性最终，我们报告了非核心SV40大T形成了一个复杂与主要热休克蛋白HSP70，我们讨论了可能的影响。

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