首页> 外文期刊>Molecular and Cellular Biology >Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae.
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Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae.

机译:BCY1的克隆与表征,一种在酿酒酵母中编码循环AMP依赖性蛋白激酶的调节亚基的基因座。

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摘要

We have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase. The encoded protein has a structural organization similar to that of the RI and RII regulatory subunits of the mammalian cyclic AMP-dependent protein kinase. Strains of S. cerevisiae with disrupted BCY1 genes do not display a cyclic AMP-dependent protein kinase in vitro, fail to grow on many carbon sources, and are exquisitely sensitive to heat shock and starvation.
机译:我们克隆了来自酵母酿酒酵母的基因(BCY1),其编码了循环依赖性蛋白激酶的调节亚基。编码蛋白质具有类似于哺乳动物循环AMP依赖性蛋白激酶的RI和RII调节亚基的结构组织。具有中断的BCY1基因的酿酒酵母的菌株在体外没有显示循环依赖性蛋白激酶,不能在许多碳源上生长,并且对热休克和饥饿是敏感的敏感性。

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