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首页> 外文期刊>Environmental engineering and management journal >PHOTOAUTOTROPHIC PRODUCTION OF POLY-B-HYDROXYBUTYRATE (PHB) FROM CYANOBACTERIA: NITRATE EFFECTS AND SCREENING OF STRAINS
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PHOTOAUTOTROPHIC PRODUCTION OF POLY-B-HYDROXYBUTYRATE (PHB) FROM CYANOBACTERIA: NITRATE EFFECTS AND SCREENING OF STRAINS

机译：蓝细菌的光养性生产聚-B-羟基丁酸酯（PHB）：硝酸盐作用和菌株筛选

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The polyhydroxybutyrate (PHB) production by photoautotrophic cultures is a potential eco-sustainable process to produce bioplastics. The biosynthesis of PHB by cyanobacteria meets a twofold target: the use of CO_2 as feedstock alternative to the fossil resource used in the conventional processes; the contribution to the reduction of the CO_2 concentration in the atmosphere. This contribution reports results of a study regarding the optimal growth conditions for cyanobacteria to produce PHB under photoautotrophic conditions using 16h/8h light/dark irradiation strategy. Synechocystis PCC6803 was selected as the model organism for optimization of the medium composition with respect to PHB production. Four culture media, characterized by different nitrate concentrations, were investigated: BG11 (optimal nitrate concentration), BG_(1/2) (half of optimal nitrate concentration), BG_(1/4) (one fourth of the optimal nitrate concentration) and BGo (nitrogen-starved conditions). BG_(1/2) proofed to be the best medium to optimize the PHB production in terms of PHB fraction of cell (8% _(DCW)) and PHB productivity (7g/Ld). Five strains of cyanobacteria were then compared to select the best strain to produce PHB. Synechocystis PCC6803 and Synechocystis aquatilis were the best strains for PHB production. A PHB fraction and productivity of about 8% _(DCW) and 7g/Ld were obtained for both strains. The production performance was promising when considering the free substrate (CO_2) used.
机译：通过光合自养培养法生产聚羟基丁酸酯（PHB）是生产生物塑料的潜在生态可持续过程。蓝细菌对PHB的生物合成达到了两个目标：使用CO_2作为常规工艺中使用的化石资源的替代原料；对减少大气中CO_2浓度的贡献。该贡献报告了有关使用16h / 8h光照/黑暗照射策略在光养养条件下蓝细菌生产PHB的最佳生长条件的研究结果。选择集胞藻PCC6803作为模型生物，以优化有关PHB产生的培养基组成。研究了四种以不同硝酸盐浓度为特征的培养基：BG11（最佳硝酸盐浓度），BG_（1/2）（最佳硝酸盐浓度的一半），BG_（1/4）（最佳硝酸盐浓度的四分之一）和BGo（缺氮条件）。 BG_（1/2）被证明是优化PHB生产的最佳培养基，就细胞的PHB分数（8％_（DCW））和PHB生产率（7g / Ld）而言。然后比较了五种蓝细菌菌株，以选择产生PHB的最佳菌株。蓝藻PCC6803和水蓝藻是生产PHB的最佳菌株。两种菌株的PHB分数和生产率分别约为8％_（DCW）和7g / Ld。当考虑使用的游离底物（CO_2）时，生产性能令人鼓舞。

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