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首页> 外文期刊>Environmental Engineering Science >Anaerobic Phenol Oxidation by Geobacter metallireducens Using Various Electron Acceptors
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Anaerobic Phenol Oxidation by Geobacter metallireducens Using Various Electron Acceptors

机译:使用各种电子受体的金属还原杆菌对厌氧苯酚的氧化

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The oxidation of phenol by the dissimilatory metal-reducing bacterium Geobacter metallireducens was studied with various terminal electron acceptors (TEAs) including: nitrate, ferric citrate, hematite (α-Fe_2O_3), pyrolusite (β-MnO_2), and birnessite (δ-MnO_(1.8)). G. metallireducens (2 x 10~8 cells mL~(-1)) oxidized phenol (0.10 mM) as the sole electron donor with ferric citrate (25 mM) as the TEA in less than 3 days, but required over 30 days with nitrate (3 mM). Only 0.03 mM phenol was oxidized with hematite (2 g L~(-1)) as the TEA after 45 days. The electron shuttling compound anthraquinone-2,6-disulfonate (AQDS; 25 μM) was added to hematite suspensions and slightly increased the 45-day extent of phenol oxidation. No phenol was oxidized with pyrolusite (2 g L~(-1)). Abiotic oxidation of phenol by birnessite (2 g L~(-1)) required testing at a lower concentration (0.2 g L~(-1)). Phenol was completely oxidized with birnessite as the TEA after 30 days, while ca. 15% of the phenol was oxidized in corresponding abiotic controls.
机译:用多种末端电子受体(TEA)研究了异化金属还原细菌Geobacter metallireducens对苯酚的氧化反应,这些受体包括:硝酸盐,柠檬酸铁,赤铁矿(α-Fe_2O_3),软锰矿(β-MnO_2)和水钠锰矿(δ-MnO_ (1.8))。 G.metallireducens(2 x 10〜8个细胞mL〜(-1))在不到3天的时间内用柠檬酸铁(25 mM)作为TEA氧化了苯酚(0.10 mM)作为唯一的电子供体,但需要30天以上的时间硝酸盐(3 mM)。 45天后,仅有0.03 mM苯酚被赤铁矿(2 g L〜(-1))氧化为TEA。将电子穿梭化合物蒽醌-2,6-二磺酸盐(AQDS; 25μM)添加到赤铁矿悬浮液中,并稍微增加了45天酚氧化的程度。吡咯石(2 g L〜(-1))没有氧化苯酚。水钠锰矿(2 g L〜(-1))对苯酚的非生物氧化需要在较低浓度(0.2 g L〜(-1))下进行测试。 30天后,以水钠锰矿作为TEA将苯酚完全氧化,而15%的苯酚在相应的非生物对照中被氧化。

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