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Evaluation of applicability of the Sartorius Airport MD8 sampler for detection of Bacillus endospores in indoor air

机译:评估Sartorius Airport MD8采样器在室内空气中检测芽孢杆菌内生孢子的适用性

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摘要

This study was designed to evaluate the measuring range and lowest limit of detection of Bacillus endospores in the ambient room air when the Sartorius MD8 sampler, and two different culture methods for bacterial enumeration were used. Different concentrations of bioaerosol were generated inside the test chamber filled with either the high-efficiency particulate air (HEPA)-filtered air or with the ambient room air. The detection of endospores in the HEPA-filtered air was achievable: (1) when they were aerosolized at a concentration above 7.56× 10~3 CFU/m~3 and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 4.00 × 10~2 CFU/m3 and analyzed with pour plate method. The detection of endospores in the ambient room air was possible: (1) when they were aerosolized at a concentration above 9.1 × 10~3 CFU/m~3 and analyzed with spread plate method, and (2) when they were aerosolized at a concentration above 5.6× 102 CFU/m3 and analyzed with pour plate method. The microorganisms present in the ambient room air interfere with precise quantification of Bacillus endospores when their concentration is relatively low. The results of this study may be helpful in critical assessment of the results obtained from monitoring the air for bacterial endospores.
机译:本研究旨在评估使用Sartorius MD8采样器和两种不同的细菌计数培养方法在室内空气中检测芽孢杆菌内生孢子的测量范围和最低限度。在充满高效微粒空气(HEPA)过滤空气或环境室内空气的测试室内部产生了不同浓度的生物气溶胶。在HEPA过滤后的空气中可以检测到内生孢子:(1)当其以高于7.56×10〜3 CFU / m〜3的浓度雾化并用展板法进行分析时,(2)在浓度高于4.00×10〜2 CFU / m3,并采用倾板法进行分析。可以检测室内空气中的内生孢子:(1)当其以高于9.1×10〜3 CFU / m〜3的浓度雾化并用展板法进行分析时;(2)当它们在室温下雾化时。浓度高于5.6×102 CFU / m3,并采用倾板法进行分析。当它们的浓度相对较低时,室内空气中存在的微生物会干扰芽孢杆菌内生孢子的精确定量。这项研究的结果可能有助于对空气中细菌内生孢子的监测结果进行严格评估。

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