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首页> 外文期刊>Environmental Monitoring and Assessment >Stability of lysosomal membrane in Carcinus maenas acts as a biomarker of exposure to Pharmaceuticals
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Stability of lysosomal membrane in Carcinus maenas acts as a biomarker of exposure to Pharmaceuticals

机译：麦氏癌中溶酶体膜的稳定性是暴露于药物的生物标志

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摘要

The presence of pharmaceuticals in the environment is now a major concern given their potential adverse effects on organisms, particularly human beings. Because the feeding style and habitat of the crab Carcinus maenas make this species vulnerable to organic contaminants, it has been used previously in ecotoxicological studies. Lysosomal membrane stability (LMS) in crabs is a general indicator of cellular well-being and can be visualized by the neutral red retention (NRR) assay. LMS in crab hemolymph has been evaluated as a cellular biomarker of adverse effects produced by exposure to pharmaceutical compounds. Crabs were exposed in the laboratory to four different Pharmaceuticals for 28 days in a semistatic 24-h renewal assay. Filtered seawater was spiked every 2 days with various concentrations (from 0.1 to 50 μg·L(-1)) of caffeine, ibuprofen, carbamazepine, and novobiocin. Results showed that NRR time, measured at day 28, was significantly reduced (p<0.05) after exposure to environmental concentrations of each pharmaceutical (caffeine=15 μg·L(-1); carbamazepine= 1 μg·L(-1); ibuprofen=5 μg·L(-1); and novobiocin= 0.1μg·L(-1)) when compared with control organisms. The predicted "no environmental effect" concentration/ measured environmental concentration results showed that the selected Pharmaceuticals are toxic at environmental concentrations and need further assessment. LMS monitoring in crabs is a sensitive tool for evaluating exposure to concentrations of selected drugs under laboratory conditions and provides a robust tier 1 testing approach (screening biomarker) for rapid assessment of marine pollution and environmental impact assessments for analyzing pharmaceutical contamination in aquatic environments.
机译：考虑到药物对生物体，特别是对人类的潜在不利影响，目前环境中存在药物是一个主要问题。由于蟹Carcinus maenas的取食方式和栖息地使该物种易受有机污染物的侵害，因此先前已在生态毒理学研究中使用过它。螃蟹的溶酶体膜稳定性（LMS）是细胞健康的一般指标，可以通过中性红red留（NRR）分析来观察。螃蟹血淋巴中的LMS已被评估为通过暴露于药物化合物产生的不良反应的细胞生物标志物。在半静态的24小时更新测定中，将螃蟹在实验室中暴露于四种不同的药物28天。每两天将过滤的海水加标各种浓度（0.1至50μg·L（-1））的咖啡因，布洛芬，卡马西平和新霉素。结果显示，暴露于每种药物的环境浓度（咖啡因= 15μg·L（-1）；卡马西平= 1μg·L（-1）；在每种药物的环境浓度下，在第28天测量的NRR时间均显着减少（p <0.05）。与对照组相比，布洛芬= 5μg·L（-1）；新霉素=0.1μg·L（-1））。预测的“无环境影响”浓度/测量的环境浓度结果表明，所选药物在环境浓度下是有毒的，需要进一步评估。螃蟹的LMS监测是评估实验室条件下所选药物浓度的敏感工具，并提供了强大的1级测试方法（筛选生物标志物）来快速评估海洋污染和环境影响评估，以分析水生环境中的药物污染。

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  • 来源
    《Environmental Monitoring and Assessment》 |2013年第5期|3783-3793|共11页
  • 作者

    G. V. Aguirre-Martinez; S. Buratti; E. Fabbri; T. A. Del Vails; M. L. Martin-Diaz;

  • 作者单位

    Catedra UNESCO/UNITWIN/WiCop, Facultad de Ciencias del Mar y Ambientales, Universidad de Cadiz,Poligono Rio San Pedro s,Puerto Real, Cadiz, Spain;

    Andalusian Center of Marine Science and Technology (CACYTMAR), Campus Universitario de Puerto Real,11510 Puerto Real, Cadiz, Spain;

    Interdepartmental Centre for Research in Environmental Sciences (CIRSA), University of Bologna,Via S. Alberto 163,48123 Ravenna, Italy;

    Interdepartmental Centre for Research in Environmental Sciences (CIRSA), University of Bologna,Via S. Alberto 163,48123 Ravenna, Italy;

    Catedra UNESCO/UNITWIN/WiCop, Facultad de Ciencias del Mar y Ambientales, Universidad de Cadiz,Poligono Rio San Pedro s,Puerto Real, Cadiz, Spain;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    hemolymph; neutral red; bioassay; therapeutic drugs; crabs;

    机译：血淋巴;中性红;生物分析;治疗药物;螃蟹;

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