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首页> 外文期刊>Environmental Pollution >Proteomic analysis of Sydney Rock oysters (Saccostrea glomerata) exposed to metal contamination in the field
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Proteomic analysis of Sydney Rock oysters (Saccostrea glomerata) exposed to metal contamination in the field

机译：现场暴露于金属污染的悉尼岩石牡蛎（Saccostrea glomerata）的蛋白质组学分析

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摘要

This study used proteomics to assess the impacts of metal contamination in the field on Sydney Rock oysters. Oysters were transplanted into Lake Macquarie, NSW, for two weeks in both 2009 and 2010. Two-dimensional electrophoresis identified changes in protein expression profiles of oyster haemo lymph between control and metal contaminated sites. There were unique protein expression profiles for each field trial. Principal components analysis attributed these differences in oyster proteomes to the different combinations and concentrations of metals and other environmental variables present during the three field trials. Identification of differentially expressed proteins showed that proteins associated with cytoskeletal activity and stress responses were the most commonly affected biological functions in the Sydney Rock oyster. Overall, the data show that proteomics combined with multivariate analysis has the potential to link the effects of contaminants with biological consequences.
机译：这项研究使用蛋白质组学评估了田间金属污染对悉尼岩牡蛎的影响。将牡蛎分别于2009年和2010年移植到新南威尔士州的麦夸里湖，历时两周。二维电泳鉴定了牡蛎血淋巴的蛋白质表达谱在对照和金属污染位点之间的变化。每个田间试验都有独特的蛋白质表达谱。主成分分析将牡蛎蛋白质组中的这些差异归因于三个田间试验中存在的金属的不同组合和浓度以及其他环境变量。差异表达蛋白的鉴定表明，与细胞骨架活性和应激反应相关的蛋白是悉尼岩石牡蛎中最常受影响的生物学功能。总体而言，数据表明，蛋白质组学与多变量分析相结合，具有将污染物的影响与生物学后果联系起来的潜力。

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  • 来源
    《Environmental Pollution》 |2012年第11期|p.102-112|共11页
  • 作者

    Emma L. Thompson; Daisy A. Taylor; Sham V. Nair; Gavin Birch; Grant C. Hose; David A. Raftos;

  • 作者单位

    Department of Biological Sciences, Macquarie University, NSW 2109, Australia,Sydney Institute of Marine Science, Chowder Bay, NSW 2088, Australia;

    Department of Biological Sciences, Macquarie University, NSW 2109, Australia,Sydney Institute of Marine Science, Chowder Bay, NSW 2088, Australia;

    Department of Biological Sciences, Macquarie University, NSW 2109, Australia;

    Department of Geochemistry, University of Sydney, NSW 2006, Australia;

    Department of Biological Sciences, Macquarie University, NSW 2109, Australia;

    Department of Biological Sciences, Macquarie University, NSW 2109, Australia,Sydney Institute of Marine Science, Chowder Bay, NSW 2088, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    metal contamination; molecular biomarkers; saccostrea glomerata; sydney rock oysters; environmental proteomics;

    机译：金属污染;分子生物标志物糖球菌悉尼牡蛎;环境蛋白质组学;

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