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首页> 外文期刊>Environmental Pollution >Bioaugmentation Of A 4-chloronitrobenzene Contaminated Soil With Pseudomonas Putida Zwl73
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Bioaugmentation Of A 4-chloronitrobenzene Contaminated Soil With Pseudomonas Putida Zwl73

机译:恶臭假单胞菌Zwl73对4-氯硝基苯污染土壤的生物强化

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摘要

The strain Pseudomonas putida ZWL73, which metabolizes 4-chloronitrobenzene (4CNB) by a partial-reductive pathway, was inoculated into lab-scale 4CNB-contaminated soil for bioaugmentation purposes in this study. The degradation of 4CNB was clearly stimulated, as indicated with the gradual accumulation of ammonium and chloride. Simultaneously, the diversity and quantity of cultivable heterotrophic bacteria decreased due to 4CNB contamination, while the quantity of 4CNB-resistant bacteria increased. During the bioaugmentation, denaturing gradient gel electrophoresis analysis showed the changes of diversity in dominant populations of intrinsic soil microbiota. The results showed that Alphaproteobac-teria and Betaproteobacteria were not distinctly affected, but Actinobacteria were apparently stimulated. In addition, an interesting dynamic within Acidobacteria was observed, as well as an influence on ammonia-oxidizing bacteria population. These combined findings demonstrate that the removal of 4CNB in soils by inoculating strain ZWL73 is feasible, and that specific populations in soils rapidly changed in response to 4CNB contamination and subsequent bioaugmentation.
机译:通过部分还原途径代谢4-氯硝基苯(4CNB)的恶臭假单胞菌ZWL73菌株被接种到实验室规模受4CNB污染的土壤中,用于生物强化目的。铵和氯化物的逐渐积累表明,明显促进了4CNB的降解。同时,由于4CNB污染,可培养的异养细菌的多样性和数量减少,而对4CNB耐药的细菌数量增加。在生物强化过程中​​,变性梯度凝胶电泳分析显示了固有土壤微生物群落优势种群多样性的变化。结果表明,α-变形杆菌和β-变形杆菌没有受到明显的影响,但放线菌明显受到了刺激。此外,在酸性细菌内观察到有趣的动态,以及对氨氧化细菌种群的影响。这些综合发现表明,通过接种菌株ZWL73去除土壤中的4CNB是可行的,并且土壤中的特定种群响应于4CNB污染和随后的生物强化而迅速变化。

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