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Characterizations of microbial diversity and machine oil degrading microbes in machine oil contaminated soil

机译:微生物多样性和机油降解微生物在机油污染土壤中的特征

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摘要

Microbial diversity in machine oil contaminated soil was determined by high-throughput amplicon sequencing technology. The diversity of culturable microbes in the contaminated soil was further characterized using polymerase chain reaction method. Proteobacteria and Bacteroidetes were the most dominant phyla and occupied 52.73 and 16.77%, respectively, while the most abundant genera were Methylotenera (21.62%) and Flavobacterium (3.06%) in the soil. In the culturable microbes, the major phyla were Firmicutes (46.15%) and Proteobacteria (37.36%) and the most abundant genera were Bacillus (42.86%) and Aeromonas (34.07%). Four isolated microbes with high machine oil degradation efficiency were selected to evaluate their characteristics on the oil degradation. All of them reached their highest oil degradation rate after 7 days of incubation. Most of them significantly increased their oil degradation rate by additional carbon or organic nitrogen source in the incubation medium. The oil degradation rate by combination of the four microbes at the same inoculation level was also higher than the rate from each individual microbe. The protocol and findings of this study are very useful for developing micro-bioremediation method to eliminate machine oil contaminants from soil. (C) 2019 Elsevier Ltd. All rights reserved.
机译:通过高通量扩增子测序技术确定机油受污染土壤中的微生物多样性。使用聚合酶链反应方法进一步表征污染土壤中培养微生物的多样性。植物体外和细菌性分别是最占优势的植物,分别占据52.73和16.77%,而最丰富的属是土壤中的甲基(21.62%)和黄杆菌(3.06%)。在培养的微生物中,主要的培养基是迫切的(46.15%)和植物体外(37.36%),最丰富的白芽孢杆菌(42.86%)和航空炎(34.07%)。选择具有高机油降解效率的四种分离的微生物以评估它们对油降解的特性。孵化7天后,所有这些都达到了最高的油退化率。其中大多数通过孵育介质中的额外碳或有机氮源显着提高了其油降解速率。通过在相同的接种水平下组合的油降解率也高于每种单层微生物的速率。该研究的方案和结果对于开发微生物化方法,非常有用,以消除土壤中的机油污染物。 (c)2019 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Environmental Pollution》 |2019年第1期|113190.1-113190.10|共10页
  • 作者单位

    Shaanxi Univ Technol Sch Biol Sci & Engn Hanzhong Shaanxi Peoples R China|Shaanxi Univ Technol Shaanxi Prov Engn Res Ctr Edible & Med Microbes Hanzhong Shaanxi Peoples R China|Shaanxi Univ Technol Shaanxi Key Lab Bioresources Hanzhong Shaanxi Peoples R China;

    Shaanxi Univ Technol Sch Biol Sci & Engn Hanzhong Shaanxi Peoples R China|Shaanxi Univ Technol Shaanxi Prov Engn Res Ctr Edible & Med Microbes Hanzhong Shaanxi Peoples R China;

    Shaanxi Univ Technol Sch Biol Sci & Engn Hanzhong Shaanxi Peoples R China;

    Shaanxi Univ Technol Sch Biol Sci & Engn Hanzhong Shaanxi Peoples R China|Shaanxi Univ Technol Shaanxi Key Lab Bioresources Hanzhong Shaanxi Peoples R China;

    Louisiana State Univ Sch Nutr & Food Sci Baton Rouge LA 70803 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Microbes; Machine oil; Contamination; Pollution; Petroleum; Degradation;

    机译:微生物;机油;污染;污染;石油;退化;

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