Silicone stressed response of crayfish (Procambarus clarkii) in antioxidant enzyme activity and related gene expression

Hossain Md Muzammel; Huang Hengliang; Yuan Yuan; Wan Tianyin; Jiang Chengfeng; Dai Zhihua; Xiong Shuanglian; Cao Menghua; Tu Shuxin

首页> 外文期刊>Environmental Pollution >Silicone stressed response of crayfish (Procambarus clarkii) in antioxidant enzyme activity and related gene expression

【24h】

Silicone stressed response of crayfish (Procambarus clarkii) in antioxidant enzyme activity and related gene expression

机译：丘疹（Procambarus clarkii）在抗氧化酶活性和相关基因表达中的硅胶胁迫反应

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Organosilicon has been widely used in various fields of industry and agriculture due to its excellent properties, such as high and low temperature resistance, flame retardant, insulation, radiation resistance and physiological inertia. However, organosilicon toxicity in aquatic animals is seldom known. In this research, two typical silicone or silane coupling agents (KH-560 (3-Glycidoxypropyltrimethoxysilane) and KH-570 (3-Methacryloxypropyltrimethoxysilane)) were used in a hydroponic experiment to evaluate the effects on survival rate, antioxidant response and gene expression in red swamp crayfish (Procambarus clarkii). Crayfishes were grown in black aquaculture boxes containing different concentrations (0, 10, 100 and 1000 mg L-1) of KH-560 and KH-570 for 72 h, and then crayfish samples were harvested and separated into tissues of carapace, gill and muscle for analysis. The results showed that silicone significantly increased malondialdehyde (MDA) content in muscle by 17%-38% except for the treatment of 100 mg L-1 KH-570, and reduced the survival rate of crayfish. Additionally, silicone KH-570 increased the activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) by 15%-31%, 17%-35%, and 9%-46%, as well as the contents of ascorbate (AsA) and glutathione (GSH) by 19%-31%, and 23%-29% respectively, in muscle tissue, and similar results occurred in KH-560. In the carapace, however, SOD activity was significantly decreased at high concentrations level of both silicone treatments. Moreover, silicon (Si) content was higher in the abdominal muscle of crayfish after silicone treatment. Assay of gene expression showed an obvious increasing expression of antioxidant related genes (Sod1, Sod2, Cat1, Cat2, and Pod1, Pod2) under silicone stress. The above results suggested that silicone caused an obvious stress response in crayfish in both biochemical and molecular levels. (C) 2020 Elsevier Ltd. All rights reserved.

机译：有机硅已经由于其优异的性能，如高和耐低温性，阻燃，绝缘，耐辐射性和生理惰性被广泛地应用于工业和农业的各个领域。然而，在水产动物有机硅毒性鲜为人知。在此研究中，两个典型的硅氧烷或硅烷偶联剂（KH-560（3-环氧丙氧基丙基）和KH-570（3-甲基丙烯酰））在水培试验中使用，以评估对存活率，抗氧化反应和基因表达中的影响沼泽红螯虾（螯虾）。小龙虾在含有不同浓度的黑色的水产养殖箱中生长（0，10，100和1000毫克的L-1）的KH-560和KH-570 72小时，然后小龙虾收获样品和分离成甲壳，鳃的组织和肌肉进行分析。结果表明在肌肉硅酮显著增加丙二醛（MDA）的含量由17％-38％，除了将100mg L-1 KH-570的治疗，并减少小龙虾的存活率。此外，有机硅KH-570增加的超氧化物歧化酶（SOD），过氧化氢酶（CAT）和过氧化物酶（POD）由15％-31％，17％-35％，的活动和9％-46％，以及内容抗坏血酸（ASA）和谷胱甘肽（GSH）由19％-31％，和23％-29％，分别在肌肉组织，和类似的结果发生在KH-560。在甲壳，但是，SOD活性显著在两个硅处理高浓度的水平降低。此外，硅（Si）含量在小龙虾的有机硅处理后的腹部肌肉更高。基因表达的测定显示的硅酮应力下的抗氧化剂相关基因（SOD1，SOD2，CAT1，CAT2和POD1，POD2）一个明显的增加的表达。上述结果表明，硅酮引起生物化学和分子水平的小龙虾一个明显的应激反应。（c）2020 elestvier有限公司保留所有权利。

著录项

来源
《Environmental Pollution》 |2021年第4期|115836.1-115836.11|共11页
作者
Hossain Md Muzammel; Huang Hengliang; Yuan Yuan; Wan Tianyin; Jiang Chengfeng; Dai Zhihua; Xiong Shuanglian; Cao Menghua; Tu Shuxin;
展开▼
作者单位

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China;

Huazhong Agr Univ Coll Resources & Environm Wuhan 430070 Peoples R China|Hubei Res Ctr Soil Remediat Engn Wuhan 430070 Peoples R China;

展开▼
收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
KH-560; KH-570; Oxidative damage; Crayfish; Gene transcription;

机译：KH-560;KH-570;氧化损伤;小龙虾;基因转录;

Silicone stressed response of crayfish (Procambarus clarkii) in antioxidant enzyme activity and related gene expression

摘要

著录项

相关主题

期刊订阅