Sensitive immunoassay for simultaneous determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether: An effective and reliable strategy to estimate the typical tetrabromobisphenol A derivative and byproduct in aquatic environments

Zhang Zhen; Zhu Nuanfei; Huang Menglu; Liang Yaru; Zeng Kun; Wu Xiangyang; Liu Zhenjiang; Ma Qianchi; Qu Guangbo; Shi Jianbo

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Sensitive immunoassay for simultaneous determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether: An effective and reliable strategy to estimate the typical tetrabromobisphenol A derivative and byproduct in aquatic environments

机译：同时测定四溴双酚A双（2-羟乙基）醚和四溴双酚A单（羟乙基）醚的灵敏免疫分析：一种有效且可靠的策略，用于估算水生环境中典型的四溴双酚A衍生物和副产物

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Tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA DHEE), as one of the TBBPA derivatives, is frequently applied as a flame retardant. To date, however, little evidence has been gathered regarding its environmental behavior and fate. This is mainly attributed to the lack of an analytical approach available to measure it Mass spectrometry cannot determine levels of TBBPA DHEE. LC-MS could not ionize TBBPA DHEE to yield enough fragments, while GC-MS could not be applied for the analysis either, due to the thermal degradation of TBBPA DHEE. We here designed a novel hapten, then conjugated it with a carrier protein and produced a type of polyclonal antibody capable of recognizing TBBPA DHEE. Based on this approach, a sensitive and high-throughput indirect competitive enzyme-linked immunosorbent assay (ELISA) was established. The respective detection limit (LOD, based on 90%B/B-0) and IC50 calculated from the standard curve were 0.702 and 9.868 ng/mL for TBBPA DHEE. And the established method showed good tolerance to various matrix interferents. Using our ELISA, we measured the concentrations of these substances from environmental water samples with no pretreatment other than filtration. We found the presence of TBBPA DHEE in environmental water samples collected in Jiangsu province, and the detected concentrations ranging from 1.5 to 7.7 ng/mL. Our proposed method here could be applied to completely and systematically investigate TBBPA DHEE and TBBPA MHEE concentrations in environmental samples. (C) 2017 Elsevier Ltd. All rights reserved.

机译：作为TBBPA衍生物之一的四溴双酚A双（2-羟乙基）醚（TBBPA DHEE）通常用作阻燃剂。然而，迄今为止，关于其环境行为和命运的证据很少。这主要归因于缺乏可用于测量它的分析方法。质谱无法确定TBBPA DHEE的水平。 LC-MS不能电离TBBPA DHEE产生足够的碎片，而GC-MS也不能用于TBBPA DHEE的热降解。我们在这里设计了一种新型的半抗原，然后将其与载体蛋白缀合，并产生了一种能够识别TBBPA DHEE的多克隆抗体。基于这种方法，建立了一种灵敏且高通量的间接竞争酶联免疫吸附测定（ELISA）。根据标准曲线计算得出的TBBPA DHEE的检出限（LOD，基于90％B / B-0）和IC50为0.702和9.868 ng / mL。建立的方法对各种基质干扰物具有良好的耐受性。使用我们的ELISA，我们无需过滤就无需预处理即可测量环境水样品中这些物质的浓度。我们在江苏省收集的环境水样品中发现了TBBPA DHEE，检测到的浓度范围为1.5至7.7 ng / mL。我们在此提出的方法可用于全面，系统地研究环境样品中的TBBPA DHEE和TBBPA MHEE浓度。（C）2017 Elsevier Ltd.保留所有权利。

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来源
《Environmental Pollution》 |2017年第10期|431-438|共8页
作者
Zhang Zhen; Zhu Nuanfei; Huang Menglu; Liang Yaru; Zeng Kun; Wu Xiangyang; Liu Zhenjiang; Ma Qianchi; Qu Guangbo; Shi Jianbo;
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Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China;

Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China;

Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China;

Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, POB 2871, Beijing 100085, Peoples R China;

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收录信息美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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1. A novel and sensitive chemiluminescence immunoassay based on AuNCs@pepsin@luminol for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether [J] . Zhang Zhen, Zhu Nuanfei, Zou Yanmin, Analytica chimica acta . 2018,第期

机译：基于Auncs @ Pepsin @ Luminol的一种新颖且敏感的化学发光免疫测定，用于同时检测四溴二苯酚A双（2-羟乙基）醚和四溴二苯酚A单（羟乙基）醚
2. An ultrasensitive competitive immunosensor using silica nanoparticles as an enzyme carrier for simultaneous impedimetric detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether [J] . Zhang Zhen, Dong Shuaibing, Ge Daohan, Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2018,第期

机译：使用二氧化硅纳米粒子作为酶载体的超敏竞争性免疫传感器，用于同时阻碍四溴二苯酚A双（2-羟基乙基）醚和四溴二苯酚A单（羟乙基）醚
3. High-throughput chemiluminescence immunoassay based on Co~(2+)/ hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments [J] . Lantian Gu, Yanmin Zou, Yanshen Li, The Science of the Total Environment . 2020,第Apra20期

机译：基于Co〜（2 +）/血红素协同催化的高通量化学发光免疫分析法用于环境中四溴双酚A双（2-羟乙基）醚的灵敏检测
4. Study of Flammability and Blooming of Tetrabromobisphenol A Bis(2,3-dibromopropyl ether) Flame Retardant Polypropene with Nano-materials added [C] . LI Xiang-mei, YANG Rong-jie, LI Xiao-dong Annual SINO-American Joint International Fire Safety Conference . 2006

机译：用纳米材料加入纳米（2,3-二溴丙基醚）阻燃聚丙烯的燃烧性和膨胀的研究
5. Embryonic Exposure to Tetrabromobisphenol A and its metabolites Bisphenol A and Tetrabromobisphenol A dimethyl ether disrupts normal zebrafish (Danio rerio) development and matrix metalloproteinase expression [O] . Jessica M. McCormick, Michael S. Paiva, Max M. Häggblom, -1

机译：胚胎暴露于四溴二苯酚A及其代谢物双酚A和四溴二苯酚的二甲醚破坏了正常的斑马鱼（Danio Rerio）发育和基质金属蛋白酶表达
6. Identification of Tetrabromobisphenol A Allyl Ether and Tetrabromobisphenol A 2,3-Dibromopropyl Ether in the Ambient Environment near a Manufacturing Site and in Mollusks at a Coastal Region [O] . Qu Guangbo, Liu Aifeng, Thanh Wang, 2013

机译：在生产基地附近的沿海环境和沿海地区的软体动物中鉴定四溴双酚A烯丙基醚和四溴双酚A 2,3-二溴丙基醚
7. Twenty-eight Day Toxicity Study in Rats with Bis(2-hydroxyethyl Ether) of Tetrabromobisphenol A with Cover Letter dated 112890. [R] . 1990

机译：用双（2-羟乙基醚）四溴双酚a对大鼠进行28天毒性研究，封面信函日期为112890。

Sensitive immunoassay for simultaneous determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether: An effective and reliable strategy to estimate the typical tetrabromobisphenol A derivative and byproduct in aquatic environments

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