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Whole-cell bioreporters and risk assessment of environmental pollution: A proof-of-concept study using lead

机译：全细胞生物报告者和环境污染风险评估：使用铅的概念验证研究

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摘要

As the world burden of environmental contamination increases, it is of the utmost importance to develop streamlined approaches to environmental risk assessment in order to prioritize mitigation measures. Whole-cell biosensors or bioreporters and speciation modeling have both become of increasing interest to determine the bioavailability of pollutants, as bioavailability is increasingly in use as an indicator of risk. Herein, we examine whether bioreporter results are able to reflect expectations based on chemical reactivity and speciation modeling, with the hope to extend the research into a wider framework of risk assessment. We study a specific test case concerning the bioavailability of lead (Pb) in aqueous environments containing Pb-complexing ligands. Ligands studied include ethylene diamine tetra-acetic acid (EDTA), meso-2,3 dimercaptosuccinic acid (DMSA), leucine, methionine, cysteine, glutathione, and humic acid (HA), and we also performed experiments using natural water samples from Lake Tai (Taihu), the third largest lake in China. We find that EDTA, DMSA, cysteine, glutathione, and HA amendment significantly reduced Pb bioavailability with increasing ligand concentration according to a log-sigmoid trend. Increasing dissolved organic carbon in Taihu water also had the same effect, whereas leucine and methionine had no notable effect on bioavailability at the concentrations tested. We find that bioreporter results are in accord with the reduction of aqueous Pb2+ that we expect from the relative complexation affinities of the different ligands tested. For EDTA and HA, for which reasonably accurate ionization and complexation constants are known, speciation modeling is in agreement with bioreporter response to within the level of uncertainty recognised as reasonable by the United States Environmental Protection Agency for speciation-based risk assessment applications. These findings represent a first step toward using bioreporter technology to streamline the biological confirmation or validation of speciation modeling for use in environmental risk assessment. (C) 2017 Elsevier Ltd. All rights reserved,
机译：随着世界环境污染负担的增加，为优先考虑缓解措施，开发简化的环境风险评估方法至关重要。全细胞生物传感器或生物报告者以及物种模型对于确定污染物的生物利用度都越来越引起关注，因为生物利用度越来越多地被用作风险指标。在这里，我们检查生物报告者的结果是否能够反映基于化学反应性和物种形成模型的期望，以期将研究扩展到更广泛的风险评估框架中。我们研究了一个特定的测试案例，该案例涉及铅（Pb）在含有Pb复合配体的水性环境中的生物利用度。所研究的配体包括乙二胺四乙酸（EDTA），内消旋2,3二巯基琥珀酸（DMSA），亮氨酸，蛋氨酸，半胱氨酸，谷胱甘肽和腐殖酸（HA），我们还使用湖中的天然水样进行了实验太湖（Taihu），中国第三大湖泊。我们发现EDTA，DMSA，半胱氨酸，谷胱甘肽和HA修饰剂根据对数S形趋势会随着配体浓度的增加而显着降低Pb的生物利用度。在太湖水中增加溶解的有机碳也具有相同的作用，而亮氨酸和蛋氨酸在所测试的浓度下对生物利用度没有显着影响。我们发现生物报告的结果与从测试的不同配体的相对络合亲和力所期望的水性Pb2 +的减少是一致的。对于已知有相当准确的电离和络合常数的EDTA和HA，物种建模与生物报告者对美国环境保护署认可的基于物种风险评估的合理不确定性范围内的响应相一致。这些发现代表了朝着使用生物报告者技术简化用于环境风险评估的物种模型的生物学确认或确认迈出的第一步。 （C）2017 Elsevier Ltd.保留所有权利，

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  • 来源
    《Environmental Pollution》 |2017年第10期|902-910|共9页
  • 作者

    Zhang Xiaokai; Qin Boqiang; Deng Jianming; Wells Mona;

  • 作者单位

    Xian Jiaotong Liverpool Univ, Dept Environm Sci, Suzhou 215123, Jiangsu, Peoples R China|Univ Liverpool, Dept Environm Sci, Brownlow Hill, Liverpool L69 7ZX, Merseyside, England;

    Chinese Acad Sci, State Key Lab Lake Sci & Environm, Taihu Lab Lake Ecosyst Res, Nanjing Inst Geog & Limnol, Nanjing 210008, Jiangsu, Peoples R China;

    Chinese Acad Sci, State Key Lab Lake Sci & Environm, Taihu Lab Lake Ecosyst Res, Nanjing Inst Geog & Limnol, Nanjing 210008, Jiangsu, Peoples R China;

    Xian Jiaotong Liverpool Univ, Dept Environm Sci, Suzhou 215123, Jiangsu, Peoples R China|Univ Liverpool, Dept Environm Sci, Brownlow Hill, Liverpool L69 7ZX, Merseyside, England;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Whole-cell bioreporter; Bioavailability; Pb; Chelating agent; Organic ligands;

    机译：全细胞生物报告物;生物利用度;铅;螯合剂;有机配体;

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