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首页> 外文期刊>Environmental Pollution >Molecular characterization of antibiotic resistance in cultivable multidrug-resistant bacteria from livestock manure
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Molecular characterization of antibiotic resistance in cultivable multidrug-resistant bacteria from livestock manure

机译：畜禽粪便中可培养的多药耐药细菌的抗生素耐药性分子表征

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Diverse antibiotic-resistance genes (ARGs) are frequently reported to have high prevalence in veterinary manure samples due to extensive use of antibiotics in farm animals. However, the characteristics of the distribution and transmission of ARGs among bacteria, especially among different species of multiple antibiotic-resistant bacteria (MARE), have not been well explored. By applying high-throughput sequencing methods, our study uncovered a vast MARB reservoir in livestock manure. The genera Escherichia, Myroides, Acinetobacter, Proteus, Ignatzschineria, Alcaligenes, Providencia and Enterococcus were the predominant cultivable MARE, with compositions of 40.6%-85.7%. From chicken manure isolates, 33 MARB were selected for investigation of the molecular characteristics of antibiotic resistance. A total of 61 ARGs and 18 mobile genetic elements (MGEs) were investigated. We found that 47 ARGs were widely distributed among the 33 MARE isolates. Each isolate carried 27-36 genes responsible for resistance to eight classes of antibiotics frequently used in clinic or veterinary settings. ARGs to the six classes of antibiotics other than streptogramins and vancomycin were present in all 33 MARB isolates with a prevalence of 80%-100%. A total of 12 MGEs were widely distributed among the 33 MARE, with int11, IS26, ISaba1, and ISEcp1 simultaneously present in 100% of isolates. In addition, 9 gene cassettes within integrons and ISCR1 were detected among MARB isolates encoding resistance to different antibiotic classes. This is the first report revealing the general co-presence of multiple ARGs, various MGEs and ARG cassettes in different species of individual MARE isolates in chicken manure. The results highlight a much higher risk of ARGs spreading through livestock manure to humans than we expected. (C) 2017 Elsevier Ltd. All rights reserved.
机译：由于在牲畜中广泛使用抗生素，经常在兽医粪便样品中发现多种抗生素抗性基因（ARG）患病率很高。然而，ARGs在细菌之间，尤其是在多种抗多种抗生素的细菌（MARE）之间的分布和传播特征尚未得到很好的研究。通过应用高通量测序方法，我们的研究发现了家畜粪便中巨大的MARB库。大肠埃希菌属，Myroides，不动杆菌属，变形杆菌，Ignatzschineria，产碱菌，Providencecia和肠球菌属是主要可栽培的MARE，组成为40.6％-85.7％。从鸡粪分离物中，选择了33种MARB来研究抗生素耐药性的分子特征。共调查了61个ARG和18个移动遗传元件（MGE）。我们发现47个ARGs广泛分布在33个MARE分离株中。每个分离株均携带27-36个基因，这些基因对临床或兽医中常用的八类抗生素具有抗性。在33株MARB分离物中，除链霉菌素和万古霉素外，其他六类抗生素的ARG均占80％-100％。共有12个MGE广泛分布在33个MARE中，其中int11，IS26，ISaba1和ISEcp1同时存在于100％的分离物中。此外，在编码对不同抗生素类别具有抗性的MARB分离株中，在整合素和ISCR1中检测到9个基因盒。这是第一份揭示鸡粪中不同MARE分离株的不同物种中多种ARG，各种MGE和ARG盒一般共存的报告。结果表明，ARGs通过家畜粪便传播给人类的风险要比我们预期的高得多。 （C）2017 Elsevier Ltd.保留所有权利。

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