...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Pollution >4-Nitrophenol (PNP) inhibits the expression of estrogen receptor beta and disrupts steroidogenesis during the ovarian development in female rats
【24h】

4-Nitrophenol (PNP) inhibits the expression of estrogen receptor beta and disrupts steroidogenesis during the ovarian development in female rats

机译：4-硝基苯酚（PNP）在雌性大鼠卵巢发育过程中抑制雌激素受体β的表达并破坏类固醇生成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

4-nitrophenol (PNP), isolated from diesel exhaust particles, has estrogenic and anti-androgenic activities, and affects the hypothalamus-pituitary-gonad axis in male rats. However, the effect of PNP on the reproduction of the female rats is still unknown. The aim of the study was to investigate the effect of neonatal PNP exposure on the ovarian function of female rats. The neonatal female rats were exposed to PNP (10 mg/kg, subcutaneously injection), the ovary and serum samples were collected at postnatal day (PND) 7, 14 and 21. The results showed that the ratio of primordial and primary follicles increased whereas the ratio of antral follicles decreased in the PNP treated ovaries at PND21. Even though no abnormality was observed in cyclicity, there was a significantly delayed timing of vaginal opening in PNP treated rats. The ovarian expression of steroidogenic enzymes including StAR, P450scc, P450c17 and P450arom increased at PND14 in the PNP treated rats compared with the control rats. In consistent with the gene expression, the concentration of estradiol-17 beta showed the similar pattern. However, PNP exposure failed to cause any significant change in the expression of steroidogenic enzymes in cultured neonatal ovaries. Furthermore, PNP suppressed the expression of estrogen receptor beta (ER beta), but not estrogen receptor alpha (ER alpha), in cultured ovaries or developmental ovaries. These results suggested that PNP might directly affect the expression of ER beta in the rat ovaries, resulting in the disrupted steroidogenesis during ovarian development and the delayed puberty. (C) 2017 Elsevier Ltd. All rights reserved.
机译：从柴油机废气颗粒中分离出来的4-硝基苯酚（PNP）具有雌激素和抗雄激素活性，并影响雄性大鼠的下丘脑-垂体-性腺轴。但是，PNP对雌性大鼠生殖的影响仍然未知。这项研究的目的是调查新生儿PNP暴露对雌性大鼠卵巢功能的影响。将新生雌性大鼠暴露于PNP（10 mg / kg，皮下注射），在出生后第7、14和21天收集卵巢和血清样品。结果表明，原始卵泡和初级卵泡的比率增加，而经PNP处理的卵巢在PND21时的窦房卵泡比率降低。即使未观察到周期性异常，在PNP处理的大鼠中，阴道开放的时间也明显延迟。与对照组相比，在PNP处理的大鼠中，PND14时类固醇生成酶（包括StAR，P450scc，P450c17和P450arom）的卵巢表达增加。与基因表达一致，雌二醇-17β的浓度显示出相似的模式。但是，PNP暴露未能在培养的新生儿卵巢中引起类固醇生成酶表达的任何显着变化。此外，PNP在培养的卵巢或发育性卵巢中抑制雌激素受体β（ER beta）的表达，但不抑制雌激素受体α（ER alpha）的表达。这些结果表明，PNP可能直接影响大鼠卵巢中ERβ的表达，从而导致卵巢发育过程中类固醇​​生成受阻，青春期延迟。 （C）2017 Elsevier Ltd.保留所有权利。

著录项

  • 来源
    《Environmental Pollution》 |2017年第10期|1-9|共9页
  • 作者

    Zhang Haolin; Taya Kazuyoshi; Nagaoka Kentaro; Yoshida Midori; Watanabe Gen;

  • 作者单位

    Beijing Forestry Univ, Coll Biol Sci & Technol, Physiol Anim Lab, Beijing 100083, Peoples R China|Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan|Tokyo Univ Agr & Technol, Cooperat Dept Vet Med, Vet Physiol Lab, Fac Agr, Tokyo 1838509, Japan;

    Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan;

    Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan|Tokyo Univ Agr & Technol, Cooperat Dept Vet Med, Vet Physiol Lab, Fac Agr, Tokyo 1838509, Japan;

    Natl Inst Hlth Sci, Div Pathol, Tokyo 1588501, Japan;

    Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan|Tokyo Univ Agr & Technol, Cooperat Dept Vet Med, Vet Physiol Lab, Fac Agr, Tokyo 1838509, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    4-Nitrophenol; ER beta; Neonate; Ovary; Steroidogenesis;

    机译：4-硝基苯酚;ERβ;新生儿;卵巢;类固醇生成;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号