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Genotoxicity of intermediate frequency magnetic fields in vitro and in vivo

机译：体内外中频磁场的遗传毒性

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摘要

We assessed genotoxic effects of intermediate frequency magnetic fields (MF) in vitro and in vivo. Rat primary astrocytes were exposed for 24 h to a 7.5 kHz MF at a magnetic flux density of 30 or 300 mu T. Male C57BL/6 J mice were exposed continuously for 5 weeks to a 7.5 kHz MF at 12 or 120 mu T, and blood samples were collected for the genotoxicity assays. To evaluate possible co-genotoxicity, the in vitro experiments included combined exposure with menadione (an agent that induces mitochondrial superoxide production and DNA damage) and methyl methanesulfonate (an alkylating agent). DNA damage and DNA repair (in vitro) were measured using the alkaline Comet assay and formation of micronuclei was assessed microscopically (in vivo) or using flow cyto-metry (in vitro). The results did not support genotoxicity or co-genotoxicity of 7.5 kHz MFs at magnetic flux densities up to 300 mu T in vitro or in vivo. On the contrary, there was some evidence that exposure to 7.5 kHz MFs might reduce the level of genetic damage. Strongest indication of any biological effects was obtained from measurements of relative cell number, which was significantly and consistently increased after MF exposure in all in vitro experiments. Health implications of this finding are unclear, but it suggests that 7.5 kHz MFs may stimulate cell proliferation or suppress cell death.
机译：我们评估了体外和体内中频磁场（MF）的遗传毒性作用。将大鼠原代星形胶质细胞在30或300μT的磁通密度下暴露于7.5 kHz MF 24小时。将雄性C57BL / 6 J小鼠在12或120μT的7.5 kHz MF下连续暴露5周。收集血样用于遗传毒性测定。为了评估可能的共基因毒性，体外实验包括与甲萘醌（一种诱导线粒体超氧化物生成和DNA损伤的试剂）和甲磺酸甲酯（一种烷基化试剂）联合暴露。使用碱性彗星测定法测量DNA损伤和DNA修复（体外），并在显微镜下（体内）或使用流式细胞仪（体外）评估微核的形成。该结果不支持在体外或体内在高达300μT的磁通密度下7.5 kHz MF的遗传毒性或共遗传毒性。相反，有证据表明，暴露于7.5 kHz MF可能会降低遗传损伤的水平。从相对细胞数的测量中获得了任何生物学效应的最强迹象，在所有体外实验中，MF暴露后，其显着且持续增加。该发现对健康的影响尚不清楚，但它表明7.5 kHz MF可能刺激细胞增殖或抑制细胞死亡。

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