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首页> 外文期刊>Environmental research >PFOA and PFOS interact with superoxide dismutase and induce cytotoxicity in mouse primary hepatocytes: A combined cellular and molecular methods
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PFOA and PFOS interact with superoxide dismutase and induce cytotoxicity in mouse primary hepatocytes: A combined cellular and molecular methods

机译：PFOA和PFOS与超氧化物歧化酶相互作用并诱导小鼠原代肝细胞的细胞毒性：细胞和分子联合方法

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摘要

This study investigated the adverse effects of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) on mouse primary hepatocytes by conducting cell viability, apoptosis, intracellular oxidative stress level, superoxide dismutase (SOD), catalase (CAT) activity and glutathione level assays. It was shown that PFOA and PFOS altered antioxidant enzymes activities and triggered oxidative stress, and thus exhibited cytotoxicity to the hepatocytes. Molecular mechanisms of SOD activities were measured and structural changes were explored by isothermal titration calorimetry and multiple spectroscopy. PFOA and PFOS bind to SOD via electrostatic forces with 7.634 +/- 0.06 and 9.7 +/- 0.4 sites, respectively, leading to structural and conformational changes. The overall results demonstrated that PFOS and PFOA are able to interact with SOD directly, resulting in producing oxidative stress and induce apoptosis.
机译：这项研究通过进行细胞活力，细胞凋亡，细胞内氧化应激水平，超氧化物歧化酶（SOD），过氧化氢酶（CAT）活性和谷胱甘肽水平测定，研究了全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）对小鼠原代肝细胞的不利影响。结果表明，PFOA和PFOS改变了抗氧化酶的活性并触发了氧化应激，因此对肝细胞表现出细胞毒性。测定了SOD活性的分子机制，并通过等温滴定热分析和多重光谱法研究了结构变化。 PFOA和PFOS通过静电力分别以7.634 +/- 0.06和9.7 +/- 0.4的位点与SOD结合，导致结构和构象变化。总体结果表明，PFOS和PFOA能够直接与SOD相互作用，从而产生氧化应激并诱导细胞凋亡。

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  • 来源
    《Environmental research》 |2019年第8期|63-70|共8页
  • 作者

    Xu Mengchen; Wan Jingqiang; Niu Qigui; Liu Rutao;

  • 作者单位

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 72 Jimo Binhai Rd, Qingdao 266237, Shandong, Peoples R China;

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 72 Jimo Binhai Rd, Qingdao 266237, Shandong, Peoples R China;

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 72 Jimo Binhai Rd, Qingdao 266237, Shandong, Peoples R China;

    Shandong Univ, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, 72 Jimo Binhai Rd, Qingdao 266237, Shandong, Peoples R China;

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  • 正文语种 eng
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  • 关键词

    Perfluorooctanoic acid; Perfluorooctanesulfonic acid; Superoxide dismutase; Oxidative stress; Interaction mechanisms;

    机译：全氟辛酸;全氟辛烷磺酸;超氧化物歧化酶;氧化应激;相互作用机制;

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