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首页> 外文期刊>Environmental research >Age related micronuclei frequency ranges in buccal and nasal cells in a healthy population
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Age related micronuclei frequency ranges in buccal and nasal cells in a healthy population

机译：健康人群中颊和鼻细胞中与年龄相关的微核频率范围

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摘要

Background: Micronuclei (MNs) are extranuclear DNA-containing bodies and determining MN frequencies is a measure of genomic instability. An age-related increase in MN frequencies in lymphocytes has been quantified, but this effect has not yet been measured in nasal and buccal cells. Methods: We determined the effect of age on the MN frequency distributions in buccal and nasal cells among a sample of a general adult population in Switzerland. To maximize the power to detect an effect of age in our population study, we recruited preferentially younger and older working age adults. We harvested buccal and nasal cells from 32 young (19-36 year) and 33 working age (47-71 years) participants. The collected cells were washed, centrifuged, and stained (Feulgen) before microscopic manual counting in 2000 cells. Based on these results, we developed an age-dependent background MN frequency chart to help interpret an individual's MN frequency score as an early signal for the effect of genotoxic exposure. Results: MN frequencies were respectively 0.53‰ and 0.47‰ for buccal and nasal among the younger and 0.87‰ and 1.03‰ in the older working age group. This corresponded to a multiplicative slope of 14% and 20% per 10 years of age for buccal and nasal cells, respectively. Conclusion: Based on our study results, we are able to propose an approach for interpreting an individual's MN screening results.
机译：背景：微核（MNs）是包含核外DNA的物体，确定MN频率是衡量基因组不稳定的一种方法。淋巴细胞中MN频率与年龄相关的增加已被量化，但尚未在鼻和颊细胞中测量到这种作用。方法：我们确定了年龄对瑞士普通成年人样本中颊和鼻细胞MN频率分布的影响。为了在我们的人口研究中最大程度地检测年龄的影响，我们优先招募了年龄较小的工作年龄的成年人。我们收集了32位年轻（19-36岁）和33个工作年龄（47-71岁）参与者的颊和鼻细胞。洗涤收集的细胞，离心并染色（Feulgen），然后在显微镜下手动计数2000个细胞。基于这些结果，我们开发了年龄相关的背景MN频率图，以帮助将个体的MN频率得分解释为遗传毒性暴露影响的早期信号。结果：年轻人的颊和鼻的MN频率分别为0.53‰和0.47‰，而年龄较大的人群的MN频率分别为0.87‰和1.03‰。这对应于颊细胞和鼻细胞每10岁的乘积斜率分别为14％和20％。结论：根据我们的研究结果，我们能够提出一种解释个体的MN筛查结果的方法。

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    《Environmental research》 |2020年第1期|17.1-17.5|共5页
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    Center for Primary Care and Public Health (Unisante) Formerly IST Institute for Work and Health University of Lausanne Lausanne Epalinges Switzerland;

    Environmental Carcinogenesis Unit Ospedale Policlinico San Martino Genoa Italy;

    Center for Primary Care and Public Health (Unisante) Formerly IST Institute for Work and Health University of Lausanne Lausanne Epalinges Switzerland INRS French Institute for Research and Safety Vandoeuvre France;

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  • 正文语种 eng
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  • 关键词

    Micronucleus assay; Genotoxicity; Buccal cells; Nasal cells; Biomarker;

    机译：微核分析;遗传毒性颊细胞;鼻细胞;生物标志物;

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