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Radiobiological characteristics of descendant progeny of fish and amphibian cells that survive the initial ionizing radiation dose

机译：初始电离辐射剂量生存的鱼类和两栖细胞后代后代的放射生物学特征

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Purpose: To evaluate the development of delayed lethal mutations, the production of medium borne lethal bystander signals, and the acquirement of radiosensitive or radioresistant traits in distant descendant progeny of fish and amphibian cells surviving ionizing radiationMaterials and methods: American eel brain endothelial cells (eelB) and African clawed frog epithelial cells (A6) were initially irradiated with gamma rays at 0.5 Gy or 2 Gy. Ionizing radiation (IR)-surviving cells were grown for 27 population doublings (PDs) for eelB and 43 PDs for A6. Reproductive cell death as quantified by donogenic survival assays was used to determine the development of delayed lethal mutations, the production of medium borne lethal bystander signals, and the acquirement of radiosensitive or radioresistant traits in the progeny survivors.Results: Only medium borne bystander signals produced by 2-Gy-irradiated eelB progeny survivors at 12 PDs could reduce the clonogenic survival of the bystander reporter cells. IR-induced delayed lethal mutations occurred in irradiated eelB cells at 15-18 PDs; however, subsequently propagated progeny cells retained normal replicative abilities. No IR-induced delayed lethal mutations developed in progeny of irradiated A6 cells at up to 43 PDs. eelB progeny survivors did not develop new radiosensitive or radioresistant traits while A6 progeny survivors acquired a new radiosensitive characteristic.Conclusion: This study enriches the current literature on the radiobiological characteristics of distant surviving progeny of irradiated fish and amphibian cells and highlights cell-type/species-dependent differential responses to IR. This study is the first to examine the potential transgenerational effects of progenitor irradiation in amphibian cells.
机译：目的：为了评估延迟致命突变的发展，培养的致命旁观者信号的产生，以及获取在鱼类和两栖细胞的遥远后代后代的放射敏感性或放射性剂性状的获取，存活电离放射线材料和方法：美国鳗鱼脑内皮细胞（EELB ）和非洲爪蛙上皮细胞（A6）最初用γ射线照射0.5 Gy或2 Gy。为EELB的27种群体倍增（PDS）生长电离辐射（IR），为EELB和43 PDS为A6种植。通过膨胀性存活测定量化的生殖细胞死亡用于确定延迟致命突变的发育，培养基致致死的旁观者信号的产生，以及在后代幸存者中获取放射敏感性或放射性剂性状。结果：仅产生中等凸起的旁观者信号通过2-GY辐照的EELB后代幸存者在12个PDS下可以降低旁观者报告细胞的克隆因存活。 IR诱导的延迟致命突变在辐照的eelb细胞中发生在15-18 pds;然而，随后繁殖的后代细胞保留了正常的复制能力。在辐照的A6细胞后代，在高达43 pds的后代，没有IR诱导的延迟致死突变。 EELB后代幸存者没有发育新的放射胶囊或放射性剂性状，而A6后代幸存者获得了一种新的放射敏感性。结论：本研究丰富了目前关于辐照鱼类和两栖细胞的远处存活后代的辐射生物学特征的目前的文献，并突出细胞型/物种 - 对IR的依赖性差异响应。本研究是第一个检查两栖细胞中祖辐射的潜在转基因作用的研究。

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