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首页> 外文期刊>Environmental research >Urinary metabolites of phthalates and di-iso-nonyl cyclohexane-1,2-dicarboxylate (DINCH)-Czech mothers' and newborns' exposure biomarkers
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Urinary metabolites of phthalates and di-iso-nonyl cyclohexane-1,2-dicarboxylate (DINCH)-Czech mothers' and newborns' exposure biomarkers

机译：邻苯二甲酸盐的尿代谢物和二甲基壬基环己烷-1,2-二羧酸盐（Dinch）-czech母亲'和新生儿的暴露生物标志物

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摘要

To assess human exposure to hazardous diesters of phthalic acid and their substitute di-iso-nonyl cyclohexane-1,2-dicarboxylate (DINCH), concentrations of their metabolites in urine should be determined. For the purpose of this biomonitoring study, a quick and easy sample preparation procedure for the simultaneous determination of eight phthalate and four DINCH metabolites in urine has been implemented and validated. Following the enzymatic hydrolysis and dilution with methanol, the sample is ready for the analysis by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS). The limits of quantification of this method ranged from 0.15 to 0.4 ng/mL urine with recoveries of 60-126% and repeatability in the range of 1-11%. The validated method was subsequently used for the analysis of urine samples collected from mothers and their newborn children living in two localities of the Czech Republic (Karvina and Ceske Budejovice, 2013-2014). Median concentrations of all measured metabolites (Emetabolites) were slightly lower in the urine samples collected from children (77.7 ng/mL urine) compared to their mothers (115.3 ng/mL urine), but no correlation was found between the concentrations of target compounds in children's and mothers' urine samples. The analyte with the highest concentration was monobutyl phthalate (MBP), with the median concentration of 32.1 ng/mL urine in the urine samples collected from mothers and 17.2 ng/mL urine in the samples collected from their children. This compound was also found in almost all of the measured samples. On the other hand, mono-isononyl-cyclohexane-1,2-dicarboxylate (MINCH) was not found in any urine sample. The most contaminated samples were collected from children living in the Karvina locality (median Emetabolites 103.2 ng/ mL urine), where the mono (2-ethyl-5-carboxypentyl) phthalate (cx-MEHP) compound contributed 43% to the total content of phthalate metabolites in newborns' urine. The results from our study are comparable with concentrations of the target compounds from Norway and Germany and lower compared to the results concluded in Sweden.
机译：为了评估人体暴露于邻苯二甲酸的危险二酯及其替代二 - Iso-壬基环己烷-1,2-二羧酸酯（DINCH），应确定尿液中代谢物的浓度。出于该生物监测研究的目的，已经实施并验证了尿液中同时测定八种邻苯二甲酸盐和四种Dinch代谢物的快速且易于样的样品制备方法。在酶水解和用甲醇稀释后，样品已准备好通过超高效液相色谱法偶联至串联质谱（UHPLC-MS / MS）的分析。该方法的定量极限范围为0.15至0.4ng / ml尿液，回收率为60-126％，可重复性为1-11％。随后，验证的方法用于分析患有母亲及其新生儿的尿液样本，居住在捷克共和国的两个地方（Karvina和Ceske Budejovice，2013-2014）。与母亲（115.3ng / ml尿液）相比，所有测量的代谢物（eMetabolites）的中位浓度略低于儿童（77.7ng / ml尿液）（115.3ng / ml尿液），但在靶化合物的浓度之间没有发现相关性儿童和母亲的尿样。具有最高浓度的分析物是邻苯二甲酸单丁酯（MBP），中值浓度为32.1ng / ml尿液中的尿液样品中的尿液中的尿液中，从他们儿童收集的样品中尿液中的17.2ng / ml尿液中。在几乎所有测量的样品中也发现该化合物。另一方面，在任何尿液样品中未发现单壬醇 - 环己烷-1,2-二羧酸酯（Minch）。从患有喀维纳局部的儿童收集最多污染的样品（中位Emetabolites 103.2ng / ml尿液），其中单酸甲酯（CX-MEHP）化合物的单甲酸酯（2-乙基-5-羧基戊基）占含量的43％新生儿尿液中的邻苯二甲酸酯代谢物。我们研究的结果与挪威和德国目标化合物的浓度相比，与瑞典的结果相比，较低。

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  • 来源
    《Environmental research》 |2019年第6期|342-348|共7页
  • 作者

    Urbancova Katerina; Lankova Darina; Sram Radim J.; Hajslova Jana; Pulkrabova Jana;

  • 作者单位

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic|CAS Inst Expt Med Videnska 1083 Prague 14220 4 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

    Univ Chem & Technol Fac Food & Biochem Technol Dept Food Anal & Nutr Tech 3 Prague 16628 6 Czech Republic;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Biomonitoring; Phthalates; DINCH; Metabolites; UHPLC-MS/MS;

    机译：生物监测;邻苯二甲酸盐;DINCH;代谢物;UHPLC-MS / MS;

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